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pan Keratin (panKRT) antibody

Details for Product No. ABIN94275, Supplier: Login to see
Antigen
  • xk70a
Reactivity
Mammalian
97
19
17
7
6
2
1
1
1
1
1
1
Host
Mouse
75
30
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
6
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
49
40
34
25
21
19
16
16
13
11
5
2
2
1
1
1
Supplier
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Immunogen Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Clone C-11
Isotype IgG1
Specificity The antibody C-11 reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name Cytokeratin (Pan-Reactive)
Background Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Pathways
Application Notes Flow Cytometry: Recommended dilution: 0.5 µg/mL

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-pan Keratin (panKRT) antibody (ABIN94275) Intracellular Flow Cytometry analysis Intracellular flow cytometry analysis of cytoke...
Immunohistochemistry (IHC) image for anti-pan Keratin (panKRT) antibody (ABIN94275) Detection of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma...
Product cited in: Haun, Devaraj, Marinelli et al.: "Probing intracellular biomarkers and mediators of cell activation using nanosensors and bioorthogonal chemistry." in: ACS nano, Vol. 5, Issue 4, pp. 3204-13, 2011 (PubMed).

Bianchi, Bombelli, Raimondo et al.: "Primary cell cultures from human renal cortex and renal-cell carcinoma evidence a differential expression of two spliced isoforms of Annexin A3." in: The American journal of pathology, Vol. 176, Issue 4, pp. 1660-70, 2010 (PubMed).

Broekema, Harmsen, Koerts et al.: "Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats." in: Kidney international, Vol. 68, Issue 6, pp. 2572-81, 2005 (PubMed).

Hamakawa, Sumida, Tanioka et al.: "Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis." in: Research communications in molecular pathology and pharmacology, Vol. 101, Issue 2, pp. 115-26, 1999 (PubMed).

Kovarík, Rejthar, Lauerová et al.: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, 1989 (PubMed).

Bártek, Vojt?sek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).