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TUBG1 antibody (Tubulin, gamma 1) (C-Term)

Details for Product anti-TUBG1 Antibody No. ABIN94313, Supplier: Login to see
Antigen
  • TUBC
  • TUBG
  • TUBG3
  • gamma-tubulin
  • GCP-1
  • TUBGCP1
  • 1500010O08Rik
  • AI451582
  • AI503389
  • Tubg
  • tubgl
  • zgc:55894
  • Tubg2
  • xgam
Alternatives
anti-Human TUBG1 antibody for Immunocytochemistry
Epitope
C-Term, AA 434-449
19
18
10
8
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Pig (Porcine), Mouse (Murine), Rat (Rattus), Cow (Bovine), Chicken, Protozoa, Plant
62
12
8
5
5
2
2
2
2
2
1
1
1
1
Host
Mouse
48
20
Clonality (Clone)
Monoclonal ()
Conjugate
This TUBG1 antibody is un-conjugated
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Western Blotting (WB)
57
19
17
12
12
7
6
2
1
Supplier
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Immunogen C-terminal peptide of gamma-tubulin counjugated to KLH.
Clone TU-30
Isotype IgG1
Specificity The antibody TU-30 recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa 434-449) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name gamma-tubulin (TUBG1 Antibody Abstract)
Background The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01 % of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.
Pathways Microtubule Dynamics
Application Notes Immunocytochemistry: Recommended dilution: Purified Antibody: 1-2 µg/mL
Staining technique: (a) Fix cells for 10 min in methanol at -20 °C and for 6 min in acetone at -20 °C, (b) Fix cells directly in methanol for 10 min at -20 °C or in acetone for 10 min at -20 °C.
Incubation: 45 min in room temperature
Positive control: P-19 mouse embryonal carcinoma cell line3T3 mouse fibroblasts
Application note: The antibody TU-30 stains only fixed cells.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Immunofluorescence (IF) image for anti-TUBG1 antibody (Tubulin, gamma 1) (C-Term) (ABIN94313) Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence stai...
Immunofluorescence (IF) image for anti-TUBG1 antibody (Tubulin, gamma 1) (C-Term) (ABIN94313) Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of mouse ...
Immunofluorescence (IF) image for anti-TUBG1 antibody (Tubulin, gamma 1) (C-Term) (ABIN94313) Fig. A, B, C Immunofluorescence staining (mouse fibroblasts) Immunofluorescence stain...
Product cited in: Vulprecht, David, Tibelius et al.: "STIL is required for centriole duplication in human cells." in: Journal of cell science, 2012 (PubMed).

Löffler, Fechter, Matuszewska et al.: "Cep63 recruits Cdk1 to the centrosome: implications for regulation of mitotic entry, centrosome amplification, and genome maintenance." in: Cancer research, Vol. 71, Issue 6, pp. 2129-39, 2011 (PubMed).

Koledova, Kafkova, Kraemer et al.: "DNA damage-induced degradation of Cdc25A does not lead to inhibition of Cdk2 activity in mouse embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 3, pp. 450-61, 2010 (PubMed).

Katsetos, Reddy, Dráberová et al.: "Altered cellular distribution and subcellular sorting of gamma-tubulin in diffuse astrocytic gliomas and human glioblastoma cell lines." in: Journal of neuropathology and experimental neurology, Vol. 65, Issue 5, pp. 465-77, 2006 (PubMed).

Sulimenko, Dráberová, Sulimenko et al.: "Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 12, pp. 7243-53, 2006 (PubMed).

Libusová, Sulimenko, Sulimenko et al.: "gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms." in: Experimental cell research, Vol. 295, Issue 2, pp. 375-86, 2004 (PubMed).

Binarová, Cenklová, Hause et al.: "Nuclear gamma-tubulin during acentriolar plant mitosis." in: The Plant cell, Vol. 12, Issue 3, pp. 433-42, 2000 (PubMed).

Nováková, Dráberová, Schürmann et al.: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996 (PubMed).

Background publications Mi, Gan, Chung: "Aggresome-like structure induced by isothiocyanates is novel proteasome-dependent degradation machinery." in: Biochemical and biophysical research communications, Vol. 388, Issue 2, pp. 456-62, 2009 (PubMed).

Tibelius, Marhold, Zentgraf et al.: "Microcephalin and pericentrin regulate mitotic entry via centrosome-associated Chk1." in: The Journal of cell biology, Vol. 185, Issue 7, pp. 1149-57, 2009 (PubMed).

Haren, Stearns, Lüders: "Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components." in: PLoS ONE, Vol. 4, Issue 6, pp. e5976, 2009 (PubMed).

Mi, Gan, Cheema et al.: "Cancer preventive isothiocyanates induce selective degradation of cellular {alpha}- and {beta}-tubulins by proteasomes." in: The Journal of biological chemistry, Vol. 284, Issue 25, pp. 17039-51, 2009 (PubMed).

Wiese, Zheng: "Microtubule nucleation: gamma-tubulin and beyond." in: Journal of cell science, Vol. 119, Issue Pt 20, pp. 4143-53, 2006 (PubMed).

Dráberová, Dráberová, Surviladze et al.: "Protein tyrosine kinase p53/p56(lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells." in: International immunology, Vol. 11, Issue 11, pp. 1829-39, 1999 (PubMed).