Transferrin (Tf) antibody

Antigen: Transferrin (Tf)

Synonyms: TF, ltf, pro1557, pro2086, mgc107777, LOC692564, TRF, LOC100144362, tf, MGC64251, tf-A, tf-b, MGC64306, PRO1557, PRO2086, DKFZp781D0156, MGC139350, HP, Tf, Tfn, hpx, Cd176, AI266983, MGC102653, Trf, MGC93500, gavi, cb285, sb:cb285, MGC112154, id:ibd3238, id:ibd3525, wu:fb57g06, wu:fb62h02, wu:fb63h10, wu:fb64h10, zgc:112154, 143958_at, TSF1, anon-EST:Posey265, DmelCG6186, CG6186, LTF, TFEW, conalbumin, tf1, DKFZp459H0229

Clonality: Monoclonal (HTF-14)

Host: Mouse

Reactivity: Human, Pig (Porcine), Rabbit

Conjugate: Un-conjugated

Application: Western Blotting (WB), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Radioimmunoassay (RIA), Immunocytochemistry (ICC)

Catalog no.: ABIN94486

Additional Information

Alternative name: Transferrin

Immunogen: Purified porcine transferrin.

Isotype: IgG1

Clone: HTF-14

Description: Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as aniron-transporter. In normal plasma, transferrin has a concentration of 25-50 µ mol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity incase of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes(holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, thereceptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes byvesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin(apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface,where apotransferrin is released upon exposure to normal pH. Internalization of labeledtransferrin thus represents an usefull approach to study endocytosis. Serum concentrationrises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatoryconditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certaintypes of cell division.

Specificity: The antibody HTF-14 recognizes an epitope located in the N-terminal domain of humanserum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the ironbinding family of proteins). It is synthesised in the liver and consists of two domains eachhaving a high affinity reversible binding site for Fe3+. This antibody does not react with: Bovine, Sheep, Dog (Canine), Equine (Horse), Other not tested.

Synonyms: TF, ltf, pro1557, pro2086, mgc107777, LOC692564, TRF, LOC100144362, tf, MGC64251, tf-A, tf-b, MGC64306, PRO1557, PRO2086, DKFZp781D0156, MGC139350, HP, Tf, Tfn, hpx, Cd176, AI266983, MGC102653, Trf, MGC93500, gavi, cb285, sb:cb285, MGC112154, id:ibd3238, id:ibd3525, wu:fb57g06, wu:fb62h02, wu:fb63h10, wu:fb64h10, zgc:112154, 143958_at, TSF1, anon-EST:Posey265, DmelCG6186, CG6186, LTF, TFEW, conalbumin, tf1, DKFZp459H0229

Application Details

Application Notes: Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml. Positive tissue: placentaImmunocytochemistryELISARIAFunctional Application: The antibody HTF-14 blocks binding of transferrin to the receptor.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Cardiovascular, Serum/Plasma Proteins, Enzymes, Metabolism

Restrictions: For Research Use only

Publications

Buartek, Viklickuy, Stratil: "Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding." in: British journal of haematology, Vol. 59, Issue 3, pp. 435-41, 1985 (PubMed).

Buartek, Viklickuy, Fran?k et al.: "Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity." in: Immunology letters, Vol. 4, Issue 5, pp. 231-5, 1982 (PubMed).

Novuakovua, Druaberovua, Schuermann et al.: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996 (PubMed).

Steere, Roberts, Byrne et al.: "Properties of a homogeneous C-lobe prepared by introduction of a TEV cleavage site between the lobes of human transferrin." in: Protein expression and purification, 2010 (PubMed).