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ATP Synthase Subunit D (AA 75-105), (Middle Region), (Subunit D) antibody

Details for Product No. ABIN950557, Supplier: Login to see
Antigen
  • ATP synthase D chain
  • mitochondrial
Epitope
AA 75-105, Middle Region, Subunit D
Reactivity
Human
Host
Rabbit
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Immunogen KLH conjugated synthetic peptide between 75-105 amino acids from the Central region of human ATP5H
Isotype Ig
Specificity This antibody reacts to ATP synthase subunit d.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity chromatography on Protein A
Background Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.Synonyms: ATP5H, Complex V subunit d, F1 F0 ATP synthase dubunit d, My032, mitochondria ATP synthase subunit d
Gene ID 10476
NCBI Accession NP_001003785
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Flow Cytometry (FACS) image for anti-ATP Synthase Subunit D (AA 75-105), (Middle Region), (Subunit D) antibody (ABIN950557) ATP5H Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) com...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ATP Synthase Subunit D (AA 75-105), (Middle Region), (Subunit D) antibody (ABIN950557) ATP5H antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin ...
Western Blotting (WB) image for anti-ATP Synthase Subunit D (AA 75-105), (Middle Region), (Subunit D) antibody (ABIN950557) ATP5H Antibody (Center) western blot analysis in HepG2 cell line lysates (35µg/lane)....
Background publications Martins-de-Souza, Gattaz, Schmitt et al.: "Proteomic analysis of dorsolateral prefrontal cortex indicates the involvement of cytoskeleton, oligodendrocyte, energy metabolism and new potential markers in schizophrenia." in: Journal of psychiatric research, Vol. 43, Issue 11, pp. 978-86, 2009 (PubMed).

Kim, Kim, Yoo et al.: "Identification of mitochondrial F(1)F(0)-ATP synthase interacting with galectin-3 in colon cancer cells." in: Cancer science, Vol. 99, Issue 10, pp. 1884-91, 2008 (PubMed).

Cross: "Molecular motors: turning the ATP motor." in: Nature, Vol. 427, Issue 6973, pp. 407-8, 2004 (PubMed).

Leyva, Bianchet, Amzel: "Understanding ATP synthesis: structure and mechanism of the F1-ATPase (Review)." in: Molecular membrane biology, Vol. 20, Issue 1, pp. 27-33, 2003 (PubMed).

Oster, Wang: "Rotary protein motors." in: Trends in cell biology, Vol. 13, Issue 3, pp. 114-21, 2003 (PubMed).