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CTF18, Chromosome Transmission Fidelity Factor 18 Homolog (S. Cerevisiae) (CHTF18) (AA 913-943), (C-Term) antibody

Details for Product No. ABIN951524, Supplier: Login to see
Antigen
  • CHTF18
  • zgc:113153
  • MGC81266
  • 6030457M03Rik
  • CTF18
  • Chl12
  • C16orf41
  • C321D2.2
  • C321D2.3
  • C321D2.4
  • CHL12
  • Ctf18
  • RUVBL
Epitope
C-Term, AA 913-943
16
15
4
4
2
2
2
1
Reactivity
Human
42
4
4
Host
Rabbit
33
9
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
37
21
6
4
3
2
2
1
Supplier
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Immunogen KLH conjugated synthetic peptide between 913-943 amino acids from the C-terminal region of human CHTF18
Isotype Ig
Specificity This antibody reacts to CHTF18.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity chromatography on Protein A
Alternative Name CHTF18 (CHTF18 Antibody Abstract)
Background CHTF18, CHTF8 (MIM 613202), and DCC1 (DSCC1, MIM 613203) are components of an alternative replication factor C (RFC) (see MIM 600404) complex that loads PCNA (MIM 176740) onto DNA during S phase of the cell cycle (Merkle et al., 2003 [PubMed 12766176], Bermudez et al., 2003 [PubMed 12930902]).Synonyms: C16orf41, CHL12, Chromosome transmission fidelity protein 18 homolog, hCTF18
Molecular Weight 107383 Da
Gene ID 63922
NCBI Accession NP_071375
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-CTF18, Chromosome Transmission Fidelity Factor 18 Homolog (S. Cerevisiae) (CHTF18) (AA 913-943), (C-Term) antibody (ABIN951524) CHTF18 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (3...
Product cited in: Farina, Shin, Kim et al.: "Studies with the human cohesin establishment factor, ChlR1. Association of ChlR1 with Ctf18-RFC and Fen1." in: The Journal of biological chemistry, Vol. 283, Issue 30, pp. 20925-36, 2008 (PubMed).

Background publications Murakami, Takano, Takeo et al.: "Stable interaction between the human proliferating cell nuclear antigen loader complex Ctf18-replication factor C (RFC) and DNA polymerase {epsilon} is mediated by the cohesion-specific subunits, ..." in: The Journal of biological chemistry, Vol. 285, Issue 45, pp. 34608-15, 2010 (PubMed).

Shiomi, Masutani, Hanaoka et al.: "A second proliferating cell nuclear antigen loader complex, Ctf18-replication factor C, stimulates DNA polymerase eta activity." in: The Journal of biological chemistry, Vol. 282, Issue 29, pp. 20906-14, 2007 (PubMed).

Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).