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DARS1 (DARS1) (AA 161-190), (N-Term) antibody

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AA 161-190, N-Term
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Immunogen KLH conjugated synthetic peptide between 161-190 amino acids from the N-terminal region of Human DARS. Genename: DARS
Isotype Ig
Specificity This antibody recognizes Human DARS (N-term).
Purification Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Alternative Name DARS1
Background Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis.Synonyms: AspRS, Aspartate-tRNA ligase, Aspartyl-tRNA synthetase cytoplasmic, Cell proliferation-inducing gene 40 protein, DARS, PIG40
Molecular Weight 57136 Da
Gene ID 1615
NCBI Accession NP_001340
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-DARS1 (DARS1) (AA 161-190), (N-Term) antibody (ABIN951837) Confocal immunofluorescent analysis of DARS1 Antibody (N-term) Cat.-No AP51185PU-N wi...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DARS1 (DARS1) (AA 161-190), (N-Term) antibody (ABIN951837) Formalin fixed and paraffin embedded human liver tissue stained with DARS1 Antibody (...
Western Blotting (WB) image for anti-DARS1 (DARS1) (AA 161-190), (N-Term) antibody (ABIN951837) Western blot analysis of DARS (arrow) using DARS1 Antibody (N-term) Cat.-No AP51185PU...
Western Blotting (WB) image for anti-DARS1 (DARS1) (AA 161-190), (N-Term) antibody (ABIN951837) Western blot analysis of DARS1 Antibody (N-term) in Jurkat cell line lysates (35ug/la...
Background publications Wu, Ma, Brown et al.: "Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening." in: Proteomics, Vol. 7, Issue 11, pp. 1775-85, 2007 (PubMed).

Sugiyama, Masuda, Shinoda et al.: "Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 6, pp. 1103-9, 2007 (PubMed).

Tu, Yan, Hood et al.: "Proteomics analysis of the interactome of N-myc downstream regulated gene 1 and its interactions with the androgen response program in prostate cancer cells." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 4, pp. 575-88, 2007 (PubMed).

Ling, Yao, Zheng et al.: "The C-terminal appended domain of human cytosolic leucyl-tRNA synthetase is indispensable in its interaction with arginyl-tRNA synthetase in the multi-tRNA synthetase complex." in: The Journal of biological chemistry, Vol. 280, Issue 41, pp. 34755-63, 2005 (PubMed).