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Glypican 2 (GPC2) (AA 136-165), (N-Term) antibody

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  • 2410016G05Rik
N-Term, AA 136-165
Human, Mouse (Murine)
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Immunogen KLH conjugated synthetic peptide between 136-165 amino acids from the N-terminal region of Human GPC2 (NP_689955.1) Genename: GPC2
Isotype Ig
Specificity Recognizes GPC2 (N-term).
Purification Protein A column followed by peptide Affinity purification
Alternative Name GPC2 (GPC2 Antibody Abstract)
Background The glypicans (GPC) constitute a family of heparan sulfate proteoglycans that are attached to the cell surface by a lycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1-GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine rich domain (CRD) with 14 invariant cysteine residues, a stalklike region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. Based on the degree of their amino acid sequence similarity, two subfamilies of glypicans have been defined. One subfamily (sharing from 35-63 % sequence homology) includes GPC1, 2, 4, and 6, while the second subfamily (sharing 54 % sequence identity) includes GPC3 and 5. Proteins between the two subfamilies also share 17-25 % sequence similarity (1-4). Glypicans are widely expressed in adult and fetal tissues. During embryonic development, the expression level of the various glypicans changes in a stage and tissue specific manner. GPC2, also known as cerebroglycan, is primarily expressed in developing neuronal tissues including the brain, spinal cord, dorsal root ganglia, and cranial nerves. It is found on the tracts of actively growing axons (5). Cell surface GPC2 binds midkine, indicating midkine GPC2 interaction may participate in neuronal cell migration and neurite outgrowth (6).Synonyms: Glypican-2
Gene ID 221914
NCBI Accession NP_689955
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Glypican 2 (GPC2) (AA 136-165), (N-Term) antibody (ABIN952586) Western blot analysis of GPC2 Antibody (N-term) Cat.-No AP51901PU-N in Mouse spleen t...
Western Blotting (WB) image for anti-Glypican 2 (GPC2) (AA 136-165), (N-Term) antibody (ABIN952586) Western blot analysis of GPC2 Antibody (N-term) Cat.-No AP51901PU-N in T47D cell line...
Background publications Song, Filmus: "The role of glypicans in mammalian development." in: Biochimica et biophysica acta, Vol. 1573, Issue 3, pp. 241-6, 2002

Kurosawa, Chen, Kadomatsu, Ikematsu, Sakuma, Muramatsu: "Glypican-2 binds to midkine: the role of glypican-2 in neuronal cell adhesion and neurite outgrowth." in: Glycoconjugate journal, Vol. 18, Issue 6, pp. 499-507, 2002

Filmus, Selleck: "Glypicans: proteoglycans with a surprise." in: The Journal of clinical investigation, Vol. 108, Issue 4, pp. 497-501, 2001

De Cat, David: "Developmental roles of the glypicans." in: Seminars in cell & developmental biology, Vol. 12, Issue 2, pp. 117-25, 2001

Simpson, Wellenreuther, Poustka, Pepperkok, Wiemann: "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing." in: EMBO reports, Vol. 1, Issue 3, pp. 287-92, 2001

Veugelers, De Cat, Ceulemans, Bruystens, Coomans, Dürr, Vermeesch, Marynen, David: "Glypican-6, a new member of the glypican family of cell surface heparan sulfate proteoglycans." in: The Journal of biological chemistry, Vol. 274, Issue 38, pp. 26968-77, 1999

Ivins, Litwack, Kumbasar, Stipp, Lander: "Cerebroglycan, a developmentally regulated cell-surface heparan sulfate proteoglycan, is expressed on developing axons and growth cones." in: Developmental biology, Vol. 184, Issue 2, pp. 320-32, 1997

FRANKLIN: "A cytochemical description of the multiplication of mengovirus in L-929 cells." in: The Journal of cell biology, Vol. 12, pp. 1-15, 1970