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Mediator Complex Subunit 16 (MED16) (AA 252-281), (Middle Region) antibody

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Synonyms THRAP5, MED16, DRIP92, TRAP95, 95kDa, A630083L04, Thrap5, Trap95
AA 252-281, Middle Region
(15), (13), (11), (6), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1)
(42), (5), (4), (3), (1)
(34), (8)
(2), (2), (2), (2), (2), (2)
Enzyme Immunoassay (EIA), Western Blotting (WB)
(39), (23), (5), (3), (1)
Pubmed 5 references available
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Quantity 0.4 mL
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen KLH conjugated synthetic peptide between 252-281 amino acids from the Central region of human MED16
Isotype Ig
Specificity This antibody recognizes Human MED16 (Center).
Purification Protein A column, followed by peptide affinity purification
Alternative Name MED16 (MED16 Antibody Abstract)
Background Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.Synonyms: DRIP92, Mediator complex subunit 16, Mediator of RNA polymerase II transcription subunit 16, THRAP5, Thyroid hormone receptor-associated protein complex 95 kDa component, Vitamin D3 receptor-interacting protein complex component DRIP92
Molecular Weight 72kd
Gene ID 10025
NCBI Accession NP_005472
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Mediator Complex Subunit 16 (MED16) (AA 252-281), (Middle Region) antibody (ABIN953369) Western blot analysis of MED16 Antibody (Center) in 293 cell line lysates (35ug/lane)...
Background publications Sato, Tomomori-Sato, Parmely et al.: "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology." in: Molecular cell, Vol. 14, Issue 5, pp. 685-91, 2004 (PubMed).

Tomomori-Sato, Sato, Parmely et al.: "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5846-51, 2004 (PubMed).

Wang, Sharma, Ren et al.: "A coregulatory role for the TRAP-mediator complex in androgen receptor-mediated gene expression." in: The Journal of biological chemistry, Vol. 277, Issue 45, pp. 42852-8, 2002 (PubMed).

Kang, Guermah, Yuan et al.: "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 5, pp. 2642-7, 2002 (PubMed).

Suzuki, Tsunoda, Sese et al.: "Identification and characterization of the potential promoter regions of 1031 kinds of human genes." in: Genome research, Vol. 11, Issue 5, pp. 677-84, 2001 (PubMed).

Catalog No. ABIN953369
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