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Platelet Factor 4 (PF4) antibody (Biotin)

Details for Product No. ABIN954176, Supplier: Log in to see
Antigen
  • CXCL4
  • PF-4
  • SCYB4
  • Cxcl4
  • Scyb4
  • Pf4a
  • RATPF4A
Reactivity
Human
77
16
14
1
Host
Mouse
66
23
15
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
18
8
5
3
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
64
62
18
15
10
8
8
7
4
4
3
2
2
2
2
1
1
1
1
1
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Immunogen Human Factor IV purified from the supernatant of Thrombin activated platelets.
Clone KKO
Isotype IgG2b
Specificity This antibody is specific for Human Platelet Factor IV/heparin complexes. AM31851BT-N (clone KKO), unlike AM31850BT-N (clone RTO) which binds PF4 alone, is specific for PF4/heparin complexes and is seen to have similar binding properties as auto-antibodies found in HIT/HITT.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Alternative Name Platelet Factor 4 / PF4 (PF4 Antibody Abstract)
Background Platelet Factor IV (PF4) is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity. Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT) is a life-threatening complication that manifests itself in a small population of patients exposed to intravenous-heparin. It is characterized by the production of PF4/heparin auto-antibodies. These auto-antibodies bind the PF4/heparin complexes and subsequently bind the FCGIIa receptor on the platelets surface through their Fc region. This activates the platelets and can initiate clot formation.Synonyms: C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4
Gene ID 5196
NCBI Accession NP_002610
UniProt P02776
Pathways
Application Notes ELISA: Use at 0.08 μg/mL following coating plate with PF4/ heparin complex. This can be done by incubating PF4 with an excess of heparin (100-200 U/mL), while coating the plate.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Suvarna, Rauova, McCracken et al.: "PF4/heparin complexes are T cell-dependent antigens." in: Blood, Vol. 106, Issue 3, pp. 929-31, 2005 (PubMed).

Arepally, Kamei, Park et al.: "Characterization of a murine monoclonal antibody that mimics heparin-induced thrombocytopenia antibodies." in: Blood, Vol. 95, Issue 5, pp. 1533-40, 2000 (PubMed).

Bharadwaj, Iino, Kontoyianni et al.: "Factor VII central. A novel mutation in the catalytic domain that reduces tissue factor binding, impairs activation by factor Xa, and abolishes amidolytic and coagulant activity." in: The Journal of biological chemistry, Vol. 271, Issue 48, pp. 30685-91, 1997 (PubMed).

Kazama, Pastuszyn, Wildgoose et al.: "Isolation and characterization of proteolytic fragments of human factor VIIa which inhibit the tissue factor-enhanced amidolytic activity of factor VIIa." in: The Journal of biological chemistry, Vol. 268, Issue 22, pp. 16231-40, 1993 (PubMed).

Wojtukiewicz, Tang, Ben-Josef et al.: "Solid tumor cells express functional \tethered ligand\" thrombin receptor."" in: Cancer research, Vol. 55, Issue 3, pp. 698-704, 1995 (PubMed).

Yamamoto, Nakagaki, Kisiel: "Tissue factor-dependent autoactivation of human blood coagulation factor VII." in: The Journal of biological chemistry, Vol. 267, Issue 27, pp. 19089-94, 1992 (PubMed).