Ubiquitin Specific Peptidase 12 (USP12) (AA 221-251), (Middle Region) antibody

Details for Product No. ABIN955485
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Antigen
Synonyms hapln3, USP12P1, usp12, Ubh1, ubh1, usp12l1, UBH1, USP12L1, USP12, sb:eu882, wu:fi09d12
Epitope
AA 221-251, Middle Region
(10), (9), (7), (7), (2), (2), (1), (1)
Reactivity
Human, Mouse (Murine)
(33), (14), (5), (1), (1), (1), (1), (1)
Host
Rabbit
(37), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3)
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
(39), (25), (7), (3), (2), (1), (1)
Pubmed 5 references available
Quantity 0.4 mL
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Catalog No. ABIN955485
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Immunogen KLH conjugated synthetic peptide between 221-251 amino acids from the Central region of human USP12
Isotype Ig
Specificity This antibody detects USP12 (Center).
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Xenopus.
Species reactivity (tested):Human, Mouse.
Purification Protein A column followed by peptide affinity purification
Alternative Name USP12
Background USP12is a deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.Synonyms: Deubiquitinating enzyme 12, UBH1, USP12L1, Ubiquitin carboxyl-terminal hydrolase 12, Ubiquitin thioesterase 12, Ubiquitin-hydrolyzing enzyme 1, Ubiquitin-specific-processing protease 12
Gene ID 219333
NCBI Accession NP_872294
Research Area Proteolysis / Ubiquitin
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % (W/V) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer.
Background publications Puente, Sánchez, Overall et al.: "Human and mouse proteases: a comparative genomic approach." in: Nature reviews. Genetics, Vol. 4, Issue 7, pp. 544-58, 2003 (PubMed).

Dunham, Matthews, Burton et al.: "The DNA sequence and analysis of human chromosome 13." in: Nature, Vol. 428, Issue 6982, pp. 522-8, 2004 (PubMed).

Cohn, Kee, Haas et al.: "UAF1 is a subunit of multiple deubiquitinating enzyme complexes." in: The Journal of biological chemistry, Vol. 284, Issue 8, pp. 5343-51, 2009 (PubMed).

Asano, Matsushita, Umeno et al.: "A genome-wide association study identifies three new susceptibility loci for ulcerative colitis in the Japanese population." in: Nature genetics, Vol. 41, Issue 12, pp. 1325-9, 2009 (PubMed).

Kee, Yang, Cohn et al.: "WDR20 regulates activity of the USP12 x UAF1 deubiquitinating enzyme complex." in: The Journal of biological chemistry, Vol. 285, Issue 15, pp. 11252-7, 2010 (PubMed).

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