alpha-2-Macroglobulin (A2M) (C-Term) antibody

Antigen: alpha-2-Macroglobulin (A2M)

Synonyms: CPAMD5, FWP007, S863-7, DKFZp779B086, Mam, A2m1, A2maa, A2MAC1, MGC114358, RATA2MAC1, A2mp, A2M, LOC733429, DKFZp459C033, LOC100221148, LOC100224071, endod, cpamd5, fwp007, s863-7, a2mb, endodermin

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN965491

Additional Information

Alternative name: A2M

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-terminal residues of human A2M (Alpha-2-macroglobulin precursor)

Description: A2M(Alpha-2-macroglobulin is able to inhibit all four classes of proteinases by a unique trapping mechanism. This protein has a peptide stretch, called the `bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thiolester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Application Details

Application Notes: ELISA, Western blotting: 1µg/ml for 2hrs.

Purification: Purified by antigen-specific affinity chromatography.

Buffer: This antibody is stored in PBS, 50% glycerol

Preservative: 0.01% Sodium Azide

Storage: Store at -20°C.

Restrictions: For Research Use only

Publications

Product: Matthijs, Devriendt, Cassiman et al.: "Structure of the human alpha-2 macroglobulin gene and its promotor." in: Biochemical and biophysical research communications, Vol. 184, Issue 2, pp. 596-603, 1992 (PubMed).

Bishop, Bell: "Assembly of the endoplasmic reticulum phospholipid bilayer: the phosphatidylcholine transporter." in: Cell, Vol. 42, Issue 1, pp. 51-60, 1985 (PubMed).

Sottrup-Jensen, Stepanik, Kristensen et al.: "Primary structure of human alpha 2-macroglobulin. V. The complete structure." in: The Journal of biological chemistry, Vol. 259, Issue 13, pp. 8318-27, 1984 (PubMed).

Zhang, Li, Martin et al.: "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry." in: Nature biotechnology, Vol. 21, Issue 6, pp. 660-6, 2003 (PubMed).