Apolipoprotein M (APOM) antibody

Antigen: Apolipoprotein M (APOM)

Synonyms: G3a, NG20, HSPC336, MGC22400, 1190010O19Rik, APOM, MGC128780, fb62d12, wu:fb62d12, g3a, ng20, hspc336, DKFZp469N144

Clonality: Monoclonal

Host: Please enquire

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Catalog no.: ABIN965579

Additional Information

Alternative name: ApoM

Gene ID: 55937

Isotype: IgG1, IgG2b

Description: ApoM(apolipoprotein M,also designated G3a or NG20),with 188-amino acid protein(about 21kDa),is an apolipoprotein and member of the lipocalin protein family. The Apo-proteins are involved in the specific binding of cellular receptors, the regulation of lipolytic enzymes, and the process of lipid exchange. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. The N-terminal region of Apo-M contains hydrophobic residues that may promote association with the phospholipid layer of lipoprotein particles. In vitro,Apo-M is glycosylated when translated in the presence of microsomes, and remains associated with the microsomes after carbonate treatment.Apo-M is expressed in liver and kidney, and is secreted into the bloodstream in HDLs,and also found in triglyceride-rich lipoproteins and LDLs.

Specificity: Ni-NTA purified full-length recombinant ApoM.trx expressed in E. Coli strain BL21 (DE3)

Application Details

Application Notes: Western Blot: 1: 500- 1: 2,000
IF: 1: 200- 1: 1,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Purification: Antibodies are purified by protein A affinity chromatography

Storage: Store at -20° C.

Restrictions: For Research Use only

Publications

Product: Xu, Dahlbäck: "A novel human apolipoprotein (apoM)." in: The Journal of biological chemistry, Vol. 274, Issue 44, pp. 31286-90, 1999 (PubMed).

Duan, Dahlbäck, Villoutreix: "Proposed lipocalin fold for apolipoprotein M based on bioinformatics and site-directed mutagenesis." in: FEBS letters, Vol. 499, Issue 1-2, pp. 127-32, 2001 (PubMed).

Zhang, Jiao, Hurtig et al.: "Expression pattern of apolipoprotein M during mouse and human embryogenesis." in: Acta histochemica, Vol. 106, Issue 2, pp. 123-8, 2004 (PubMed).

Xu, Nilsson-Ehle, Ahrén: "Correlation of apolipoprotein M with leptin and cholesterol in normal and obese subjects." in: The Journal of nutritional biochemistry, Vol. 15, Issue 10, pp. 579-82, 2004 (PubMed).