FCGR2C antibody

Catalog No. ABIN965849

Product Details anti-FCGR2C Antibody

Target: FCGR2C (Fc Fragment of IgG, Low Affinity IIc, Receptor For (CD32) (FCGR2C))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FCGR2C antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Specificity: This antibody reacts with a 40kD (39-48kD, Endo F:35 and 29kD) polymorphic transmembranous highly glycosylated glycoprotein, also known as Fc-gRII receptor, gp40.

Purification: Purified.

Immunogen: K562 and FcgRII+L cells.

Clone: B340(7-30)

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system. This antibody was quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 5-10 mg/mL in IHC. For consistent and reproducible results on formalin-fixed, paraffinembedded sections, a high temperature antigen unmasking technique is required. After the unmasking technique, rinse the slides in buffer, followed by Tis protein blocking solution.

Comment:

Recommended Positive Control: Macrophages, granulocytes, B-Granulocytess, Eosinophils.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-FCGR2C antibody (ABIN965849)

Hogg: "The structure and function of Fc receptors." in: Immunology today, Vol. 9, Issue 7-8, pp. 185-7, (1989) (PubMed).

Target Details for FCGR2C

Target: FCGR2C (Fc Fragment of IgG, Low Affinity IIc, Receptor For (CD32) (FCGR2C))

Alternative Name: Cdw32 (FCGR2C Products)

Gene ID: 2212