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Cdw32 antibody

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Antigen
Reactivity
Human
Host
Mouse
Clonality (Clone) Monoclonal ( )
Application
Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
Pubmed 3 references available
Catalog no. ABIN965849
Quantity 0.2 mg
Price
281.88 $   Plus shipping costs $45.00
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Product details

Target Details Application Details Handling References back to top
Immunogen K562 and FcgRII+L cells.
Clone
Isotype IgG1
Specificity This antibody reacts with a 40kD (39-48kD, Endo F:35 and 29kD) polymorphic transmembranous highly glycosylated glycoprotein, also known as Fc-gRII receptor, gp40.
Purification Purified.

Application Details

Target Details Handling References back to top
Application Notes Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system. This antibody was quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 5-10 mg/mL in IHC. For consistent and reproducible results on formalin-fixed, paraffinembedded sections, a high temperature antigen unmasking technique is required. After the unmasking technique, rinse the slides in buffer, followed by Tis protein blocking solution.
Comment

Recommended Positive Control: Macrophages, granulocytes, B-Granulocytess, Eosinophils.
Restrictions For Research Use only

Handling

Target Details Application Details References back to top
Concentration 0.4 mg/ml.
Buffer 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0
Preservative 0.05% Sodium Azide
Storage 2-8 °C

References

Target Details Application Details Handling back to top
Product cited in: Stuart, Trounstine, Vaux et al.: "Isolation and expression of cDNA clones encoding a human receptor for IgG (Fc gamma RII)." in: The Journal of experimental medicine, Vol. 166, Issue 6, pp. 1668-84, 1987 (PubMed).

Hogg: "The structure and function of Fc receptors." in: Immunology today, Vol. 9, Issue 7-8, pp. 185-7, 1989 (PubMed).

Ierino, Hulett, McKenzie et al.: "Mapping epitopes of human Fc gamma RII (CDw32) with monoclonal antibodies and recombinant receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 5, pp. 1794-803, 1993 (PubMed).

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