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Fc Fragment of IgG, Low Affinity IIc, Receptor For (CD32) (Gene/pseudogene) (FCGR2C) antibody

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Antigen
  • CD32
  • CD32C
  • CDW32
  • FCG2
  • FCRIIC
  • IGFR2
Reactivity
Human
47
4
2
Host
Mouse
46
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
4
4
4
4
4
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)
42
34
3
2
1
1
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Immunogen K562 and FcgRII+L cells.
Clone B340(7-30)
Isotype IgG1
Specificity This antibody reacts with a 40kD (39-48kD, Endo F:35 and 29kD) polymorphic transmembranous highly glycosylated glycoprotein, also known as Fc-gRII receptor, gp40.
Purification Purified.
Alternative Name Cdw32 (FCGR2C Antibody Abstract)
Gene ID 2212
Pathways
Application Notes Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system. This antibody was quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 5-10 mg/mL in IHC. For consistent and reproducible results on formalin-fixed, paraffinembedded sections, a high temperature antigen unmasking technique is required. After the unmasking technique, rinse the slides in buffer, followed by Tis protein blocking solution.
Comment

Recommended Positive Control: Macrophages, granulocytes, B-Granulocytess, Eosinophils.

Restrictions For Research Use only
Format Liquid
Concentration 0.4 mg/ml.
Buffer 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Hogg: "The structure and function of Fc receptors." in: Immunology today, Vol. 9, Issue 7-8, pp. 185-7, 1989 (PubMed).

Background publications Ierino, Hulett, McKenzie et al.: "Mapping epitopes of human Fc gamma RII (CDw32) with monoclonal antibodies and recombinant receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 5, pp. 1794-803, 1993 (PubMed).

Stuart, Trounstine, Vaux et al.: "Isolation and expression of cDNA clones encoding a human receptor for IgG (Fc gamma RII)." in: The Journal of experimental medicine, Vol. 166, Issue 6, pp. 1668-84, 1987 (PubMed).