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Fc Fragment of IgG, Low Affinity IIc, Receptor For (CD32) (Gene/pseudogene) (FCGR2C) antibody

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Synonyms CD32, CD32C, CDW32, FCG2, FCRIIC, IGFR2
(49), (4), (2)
(48), (1)
Clonality (Clone)
Monoclonal ()
(4), (4), (4), (4), (4), (4)
Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(44), (34), (3), (2), (1), (1)
Pubmed 3 references available
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Quantity 0.2 mg
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Immunogen K562 and FcgRII+L cells.
Clone B340(7-30)
Isotype IgG1
Specificity This antibody reacts with a 40kD (39-48kD, Endo F:35 and 29kD) polymorphic transmembranous highly glycosylated glycoprotein, also known as Fc-gRII receptor, gp40.
Purification Purified.
Alternative Name Cdw32 (FCGR2C Antibody Abstract)
Gene ID 2212
Application Notes Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system. This antibody was quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 5-10 mg/mL in IHC. For consistent and reproducible results on formalin-fixed, paraffinembedded sections, a high temperature antigen unmasking technique is required. After the unmasking technique, rinse the slides in buffer, followed by Tis protein blocking solution.

Recommended Positive Control: Macrophages, granulocytes, B-Granulocytess, Eosinophils.

Restrictions For Research Use only
Format Liquid
Concentration 0.4 mg/ml.
Buffer 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Hogg: "The structure and function of Fc receptors." in: Immunology today, Vol. 9, Issue 7-8, pp. 185-7, 1989 (PubMed).

Background publications Ierino, Hulett, McKenzie et al.: "Mapping epitopes of human Fc gamma RII (CDw32) with monoclonal antibodies and recombinant receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 5, pp. 1794-803, 1993 (PubMed).

Stuart, Trounstine, Vaux et al.: "Isolation and expression of cDNA clones encoding a human receptor for IgG (Fc gamma RII)." in: The Journal of experimental medicine, Vol. 166, Issue 6, pp. 1668-84, 1987 (PubMed).

Catalog No. ABIN965849
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