Chromodomain Helicase DNA Binding Protein 3 (CHD3) antibody

Antigen: Chromodomain Helicase DNA Binding Protein 3 (CHD3)

Synonyms: ZFH, Mi-2a, Mi2-ALPHA, Chd7, Prp7, Prp9-1, AF020312, MGC40857, 2600010P09Rik, CHD3, LOC568230, zfh, mi-2a, mi2-alpha

Clonality: Monoclonal (2G4)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA

Catalog no.: ABIN965879

Additional Information

Alternative name: CHD3

Gene ID: 1107

Immunogen: Purified recombinant fragment of human CHD3 expressed in E. Coli.

Isotype: IgG1

Clone: 2G4

Description: This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.
Synonyms: ZFH, Mi-2a, Mi2ALPHA, CHD3

Molecular Weight: 226kDa

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascitic fluid

Preservative: 0.03% sodium azide.

Storage: Store at 4°C, for long term storage, store at -20°C.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Publications

Product: Lepesheva, Virus, Waterman: "Conservation in the CYP51 family. Role of the B' helix/BC loop and helices F and G in enzymatic function." in: Biochemistry, Vol. 42, Issue 30, pp. 9091-101, 2003 (PubMed).

Goehler, Lalowski, Stelzl et al.: "A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease." in: Molecular cell, Vol. 15, Issue 6, pp. 853-65, 2004 (PubMed).

Burd, Kinyamu, Miller et al.: "UV radiation regulates Mi-2 through protein translation and stability." in: The Journal of biological chemistry, Vol. 283, Issue 50, pp. 34976-82, 2008 (PubMed).