Keratin 17 (KRT17) antibody

Antigen: Keratin 17 (KRT17)

Synonyms: PC, K17, PC2, PCHC1, Krt1-17, MGC159887, Ka17, KRT17, krt16

Clonality: Monoclonal (3B12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Catalog no.: ABIN965900

Additional Information

Alternative name: CK17

Gene ID: 3872

Immunogen: Purified recombinant fragment of CK17 expressed in E. Coli.

Isotype: IgG2b

Clone: 3B12

Description: CK17, also known as KRT17, it is the type I intermediate filament chain keratin 17. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial stem cells. May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair.
Synonyms: PC, K17, PC2, PCHC1, KRT17

Molecular Weight: 49kDa

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascitic fluid

Preservative: 0.03% sodium azide.

Storage: Store at 4°C, for long term storage, store at -20°C.

Restrictions: For Research Use only

Publications

Product: Leigh, Navsaria, Purkis et al.: "Keratins (K16 and K17) as markers of keratinocyte hyperproliferation in psoriasis in vivo and in vitro." in: The British journal of dermatology, Vol. 133, Issue 4, pp. 501-11, 1995 (PubMed).

van de Rijn, Perou, Tibshirani et al.: "Expression of cytokeratins 17 and 5 identifies a group of breast carcinomas with poor clinical outcome." in: The American journal of pathology, Vol. 161, Issue 6, pp. 1991-6, 2002 (PubMed).