Clock Homolog (Mouse) (CLOCK) (N-Term) antibody

Antigen: Clock Homolog (Mouse) (CLOCK)

Synonyms: KAT13D, bHLHe8, KIAA0334, mKIAA0334, 5330400M04Rik, clk, Xclk, XClock, CLOCK, DKFZp469L011, zCLOCK1, zfCLOCK1, CLK, Jerk, Jrk, PAS1, dCLK, dCLK/JRK, dCLOCK, dClck, DmelCG7391, CG7391

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN965906

Additional Information

Alternative name: CLOCK

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to N-terminal residues of human CLOCK(Circadian locomoter output cycles protein kaput)

Description: CLOCK(Circadian locomoter output cycles protein kaput) is a circadian regulator that acts as a transcription factor. CLOCK-BMAL1 heterodimers bind to an E-box element (3'-CACGTG-5'), thereby activating transcription of PER1, and possibly of other circadian clock proteins. Mutant CLOCK and BMAL1 form hetereodimers that bind DNA, but fail to activate transcription. CLOCK belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.

Application Details

Application Notes: ELISA, Western blotting: 1µg/ml for 2hrs.

Purification: Purified by antigen-specific affinity chromatography.

Buffer: This antibody is stored in PBS, 50% glycerol

Preservative: 0.01% Sodium Azide

Storage: Store at -20°C.

Research Area: Cardiovascular, Atherosclerosis, Transcription Factors, Metabolism

Restrictions: For Research Use only

Publications

Product: Jin, Shearman, Weaver et al.: "A molecular mechanism regulating rhythmic output from the suprachiasmatic circadian clock." in: Cell, Vol. 96, Issue 1, pp. 57-68, 1999 (PubMed).

Steeves, King, Zhao et al.: "Molecular cloning and characterization of the human CLOCK gene: expression in the suprachiasmatic nuclei." in: Genomics, Vol. 57, Issue 2, pp. 189-200, 1999 (PubMed).

Kondratov, Chernov, Kondratova et al.: "BMAL1-dependent circadian oscillation of nuclear CLOCK: posttranslational events induced by dimerization of transcriptional activators of the mammalian clock system." in: Genes & development, Vol. 17, Issue 15, pp. 1921-32, 2003 (PubMed).

Lee, Weaver, Reppert: "Direct association between mouse PERIOD and CKIepsilon is critical for a functioning circadian clock." in: Molecular and cellular biology, Vol. 24, Issue 2, pp. 584-94, 2003 (PubMed).

Shirogane, Jin, Ang et al.: "SCFbeta-TRCP controls clock-dependent transcription via casein kinase 1-dependent degradation of the mammalian period-1 (Per1) protein." in: The Journal of biological chemistry, Vol. 280, Issue 29, pp. 26863-72, 2005 (PubMed).

Moreira, Pedrazzoli, Dos Santos Coelho et al.: "Clock gene polymorphisms and narcolepsy in positive and negative HLA-DQB1*0602 patients." in: Brain research. Molecular brain research, Vol. 140, Issue 1-2, pp. 150-4, 2005 (PubMed).

Sato, Yamada, Ukai et al.: "Feedback repression is required for mammalian circadian clock function." in: Nature genetics, Vol. 38, Issue 3, pp. 312-9, 2006 (PubMed).