Deoxycytidine Kinase (DCK) (N-Term) antibody

Antigen: Deoxycytidine Kinase (DCK)

Synonyms: dck, MGC80405, DCK, Dck, dck2, MGC174279, wu:fe11h12, wu:fj05e11, zgc:109951, wu:fc15f06, zgc:101771, MGC127248, MGC117410, MGC138632

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN965975

Additional Information

Alternative name: DCK

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to N-terminal residues of human DCK (deoxycytidine kinase)

Description: Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.

Application Details

Application Notes: ELISA, Western blotting: 1µg/ml for 2hrs.

Purification: Purified by antigen-specific affinity chromatography.

Buffer: This antibody is stored in PBS, 50% glycerol

Preservative: 0.01% Sodium Azide

Storage: Store at -20°C.

Research Area: Cancer, Cell Cycle

Restrictions: For Research Use only

Publications

Product: Johansson, Brismar, Karlsson: "Human deoxycytidine kinase is located in the cell nucleus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 22, pp. 11941-5, 1997 (PubMed).

Veuger, Heemskerk, Honders et al.: "Functional role of alternatively spliced deoxycytidine kinase in sensitivity to cytarabine of acute myeloid leukemic cells." in: Blood, Vol. 99, Issue 4, pp. 1373-80, 2002 (PubMed).

Innoceta, Galluzzi, Ruzzo et al.: "Molecular basis of 2',3'-dideoxycytidine-induced drug resistance in human cells." in: Molecular and cellular biochemistry, Vol. 231, Issue 1-2, pp. 173-7, 2002 (PubMed).

Krawiec, Kierdaszuk, Shugar: "Inorganic tripolyphosphate (PPP(i)) as a phosphate donor for human deoxyribonucleoside kinases." in: Biochemical and biophysical research communications, Vol. 301, Issue 1, pp. 192-7, 2003 (PubMed).