Elf4 (C-Term) antibody

Antigen: Elf4

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966054

Additional Information

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-terminal residues of human ELF4 (ETS-related transcription factor Elf-4)

Description: ELF4 (ETS-related transcription factor Elf-4) is a transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of the bovine lysozyme gene. It acts synergistically with RUNX1 to transactivate the IL3 promoter. ELF4 also transactivates the PRF1 promoter in natural killer (NK) cells. The protein plays a role in the development and function of NK and NK T-cells and in innate immunity. ELF4 interacts via an N-terminal region with the RUNX1 Runt domain and interacts via its C-terminus with PML, this interaction enhances ELF4 transactivation of the bovine lysozyme gene.

Application Details

Application Notes: ELISA, Western blotting: 1µg/ml for 2hrs.

Purification: Purified by antigen-specific affinity chromatography.

Buffer: This antibody is stored in PBS, 50% glycerol

Preservative: 0.01% Sodium Azide

Storage: Store at -20°C.

Restrictions: For Research Use only

Publications

Product: Mao, Frank, Zhang et al.: "Functional and physical interactions between AML1 proteins and an ETS protein, MEF: implications for the pathogenesis of t(8;21)-positive leukemias." in: Molecular and cellular biology, Vol. 19, Issue 5, pp. 3635-44, 1999 (PubMed).

Suico, Koyanagi, Ise et al.: "Functional dissection of the ETS transcription factor MEF." in: Biochimica et biophysica acta, Vol. 1577, Issue 1, pp. 113-20, 2002 (PubMed).

Cho, Akbarali, Zerbini et al.: "Isoforms of the Ets transcription factor NERF/ELF-2 physically interact with AML1 and mediate opposing effects on AML1-mediated transcription of the B cell-specific blk gene." in: The Journal of biological chemistry, Vol. 279, Issue 19, pp. 19512-22, 2004 (PubMed).

Suico, Yoshida, Seki et al.: "Myeloid Elf-1-like factor, an ETS transcription factor, up-regulates lysozyme transcription in epithelial cells through interaction with promyelocytic leukemia protein." in: The Journal of biological chemistry, Vol. 279, Issue 18, pp. 19091-8, 2004 (PubMed).