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ElF4E antibody

Details for Product No. ABIN966057, Supplier: Log in to see
Antigen
Reactivity
Human, Mouse (Murine), Rat (Rattus)
8
7
5
Host
Rabbit
7
1
Clonality
Polyclonal
Conjugate
Un-conjugated
1
Application
Immunohistochemistry (IHC)
7
6
2
1
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Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).
Specificity ElF4E (Ab-209) antibody detects endogenous levels of total elF4E protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Gu, Li, Zhao et al.: "tRNASer(CGA) differentially regulates expression of wild-type and codon-modified papillomavirus L1 genes." in: Nucleic acids research, Vol. 32, Issue 15, pp. 4448-61, 2004 (PubMed).

Li, McDonald, Nassar et al.: "Clinical outcome in stage I to III breast carcinoma and eIF4E overexpression." in: Annals of surgery, Vol. 227, Issue 5, pp. 756-6l; discussion 761-3, 1998 (PubMed).

Ohlmann, Rau, Pain et al.: "The C-terminal domain of eukaryotic protein synthesis initiation factor (eIF) 4G is sufficient to support cap-independent translation in the absence of eIF4E." in: The EMBO journal, Vol. 15, Issue 6, pp. 1371-82, 1996 (PubMed).