GadE (N-Term) antibody

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Escherichia coli (E. coli)
ELISA, Western Blotting (WB)
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Immunogen Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to N-terminal residues of Escherichia coli O157:H7 gadE(Transcriptional regulator gadE)
Purification Purified by antigen-specific affinity chromatography.
Background The gadE(Transcriptional regulator gadE) regulates the expression of several genes involved in acid resistance. GadE is required for the expression of gadA and gadBC, among others, regardless of media or growth conditions. gadE binds directly to the 20 bp GAD box found in the control regions of both loci. The gadE could be involved in the regulation of the genes coding for the type III secretion system in enterohaemorragic strains. The gadE could be induced by evgA via the induction of ydeO. The gadE contains 1 HTH luxR-type DNA-binding domain.
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Bacteria
Application Notes Western Blotting (1�g/ml for 2hrs)
Restrictions For Research Use only
Format Liquid
Buffer This antibody is stored in PBS, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Background publications Tatsuno, Nagano, Taguchi, Rong, Mori, Sasakawa: "Increased adherence to Caco-2 cells caused by disruption of the yhiE and yhiF genes in enterohemorrhagic Escherichia coli O157:H7." in: Infection and immunity, Vol. 71, Issue 5, pp. 2598-606, 2003 (PubMed).

Perna, Plunkett, Burland, Mau, Glasner, Rose, Mayhew, Evans, Gregor, Kirkpatrick, Pósfai, Hackett, Klink, Boutin, Shao, Miller, Grotbeck, Davis, Lim, Dimalanta, Potamousis, Apodaca, Anantharaman, Lin et al.: "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. ..." in: Nature, Vol. 409, Issue 6819, pp. 529-33, 2001 (PubMed).

Hayashi, Makino, Ohnishi, Kurokawa, Ishii, Yokoyama, Han, Ohtsubo, Nakayama, Murata, Tanaka, Tobe, Iida, Takami, Honda, Sasakawa, Ogasawara, Yasunaga, Kuhara, Shiba, Hattori, Shinagawa: "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 8, Issue 1, pp. 11-22, 2001 (PubMed).