Solute Carrier Family 22 (Organic Cation Transporter), Member 1 (SLC22A1) (C-Term) antibody

Antigen: Solute Carrier Family 22 (Organic Cation Transporter), Member 1 (SLC22A1)

Synonyms: SLC22A2, SLC22A1, RBOCT1, OCT1, HOCT1, oct1_cds, Lx1, Oct1, Orct, Orct1, MGC14019, Roct1, MGC93570, Octn1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966708

Additional Information

Alternative name: OCT1

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-terminal residues of human OCT1 (Organic cation transporter 1)

Description: OCT1 (Organic cation transporter 1) translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine.Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase. OCT1 is widely expressed with high level in liver. Isoform 1 and isoform 2 are expressed in liver. Isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in glial cell lines. In the liver activated by HNF4A and suppressed by bile acids via NR0B2. Increased by cholesterol treatment in hepatocyte cells. OCT1 belongs to the major facilitator superfamily and organic cation transporter family.
Synonyms: SLC22A1(Solute carrier family 22 member 1)

Application Details

Restrictions: For Research Use only

Publications

Product: Zhang, Dresser, Gray et al.: "Cloning and functional expression of a human liver organic cation transporter." in: Molecular pharmacology, Vol. 51, Issue 6, pp. 913-21, 1997 (PubMed).

Gorboulev, Ulzheimer, Akhoundova et al.: "Cloning and characterization of two human polyspecific organic cation transporters." in: DNA and cell biology, Vol. 16, Issue 7, pp. 871-81, 1997 (PubMed).

Hayer, Bönisch, Brüss: "Molecular cloning, functional characterization and genomic organization of four alternatively spliced isoforms of the human organic cation transporter 1 (hOCT1/SLC22A1)." in: Annals of human genetics, Vol. 63, Issue Pt 6, pp. 473-82, 2001 (PubMed).