Wingless-Type MMTV Integration Site Family, Member 16 (WNT16) antibody

Details for Product No. ABIN967363
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Antigen
Synonyms zgc:77293, E130309I19Rik
Reactivity
Human
(30), (14), (14), (14), (12), (2)
Host
Mouse
(32)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(18), (10), (8), (8), (7)
Pubmed 5 references available
Quantity 50 µg
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Catalog No. ABIN967363
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Immunogen Human WNT16 Recombinant Protein
Clone F4-1582
Isotype IgG2b
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Components 1) 51-9000015: Purified mouse anti-human WNT16 monoclonal antibody.
Quantity: 50 µg (1 ea).
Concentration: 0.5 mg/ml.
Clone: F4-1582.
Immunogen: Human WNT16 Recombinant Protein.
Isotype: Mouse IgG2b, kappa.
Molecular Weight: 40 kDa.
Storage Buffer: Aqueous buffered solution containing Less or equal than 0.09% sodium azide.

2) 51-9000020: 697 control Lysate.
Quantity: 50 µg (1 ea).
Concentration: 1.0 mg/ml.
Storage Buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol).
Alternative Name Wnt16
Background The Wnt gene family comprises a large group of signaling proteins involved in developmental pathways in vertebrates, Drosophilia, C. elegans and other organisms. The Wnt genes were first discovered in mouse (called int-1) and later in Drosophilia (called wingless, or wg) where the signaling pathways were unravelled. The Wnt proteins are involved in various developmental processes including embryonic induction, generation of cell polarity and the specification of cell fate. The vertebrate Wnt glycoproteins number at least 16 members and initiate signaling by being secreted and a subset of these glycoproteins bind to a class of receptors, called Frizzled, of which 11 have been identified. Stimulation of the Wnt pathway causes the phosphorylation of Dishevelled, which inhibits glycogen synthase kinase -3beta (GSK3beta) and allows beta-catenin to accumulate in the cytosol. beta-catenin then translocates to the nucleus to form a complex with Tcf/LEF family of transcription factors to activate transcription. In unstimulated cells, beta-catenin forms a complex with the proteins Axin, adenomatous polyposis coli (APC), (GSK3ß) and is unstable. Studies have shown that some members of this pathway become mutated in human cancers, such as colon carcinoma and melanoma. Futhermore, studies on the WNT-16 gene, have shown that it is activated by the E2A-Pbx fusion product in acute lymphoblastoid leukemia. Wnt16 has a predicted molecular weight of 40 kDa (SWISS-PROT:Q9UBV4).
Molecular Weight 40 kDa
Research Area Stem Cells, Cancer
Application Notes Applications include western blot analysis (0.063 - 0.25 µg/ml). 697 cell lysate [50 µg (1 µg/µl)] is provided as a positive control (51-9000020).
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Wingless-Type MMTV Integration Site Family, Member 16 (WNT16) antibody Western blot analysis of Wnt16. Lysate from 697 cells was probed with anti-Wnt 16 (clone F4-1582, ABIN967363) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.063 myg/ml (lane 3). Wnt16 is identified as a band of ~40 kDa.
Product cited in: Cadigan, Nusse: "Wnt signaling: a common theme in animal development." in: Genes & development, Vol. 11, Issue 24, pp. 3286-305, 1998 (PubMed).

Wodarz, Nusse: "Mechanisms of Wnt signaling in development." in: Annual review of cell and developmental biology, Vol. 14, pp. 59-88, 1999 (PubMed).

McWhirter, Neuteboom, Wancewicz et al.: "Oncogenic homeodomain transcription factor E2A-Pbx1 activates a novel WNT gene in pre-B acute lymphoblastoid leukemia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 20, pp. 11464-9, 1999 (PubMed).

Polakis: "Wnt signaling and cancer." in: Genes & development, Vol. 14, Issue 15, pp. 1837-51, 2000 (PubMed).

Taipale, Beachy: "The Hedgehog and Wnt signalling pathways in cancer." in: Nature, Vol. 411, Issue 6835, pp. 349-54, 2001 (PubMed).

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