Transcription Factor E3 (TFE3) antibody

Antigen: Transcription Factor E3 (TFE3)

Synonyms: TFEA, RCCP2, bHLHe33, Tfe3, Tfe-3, AI851540, F830016E06Rik, RGD1559642

Clonality: Monoclonal (G138-312)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Gel Shift (GS), Immunoprecipitation (IP)

Catalog no.: ABIN967449

Additional Information

Alternative name: TFE3

Immunogen: TFE3-L Protein

Format: Liquid

Isotype: IgG1

Clone: G138-312

Description: TFE3 is a ubiquitously expressed 50 kDa transcription factor containing both basic helix-loop-helix (bHLH) and leucine zipper (ZIP) motifs. It was initially identified as a protein which bound to the µE3 site in the immunoglobulin heavy chain enhancer. In addition, it binds to the MLTF/USF site in the adenovirus major late promoter. Upon binding as a dimer, it induces a minor groove-oriented bend in the DNA, similar to other HLH proteins such as TFEB, USF, myc, and max. Binding results in transcriptional activation and is mediated through the basic region located just N-terminal to the HLH domain. Clone G138-312 recognizes TFE3. The antibody was raised against a bacterially expressed TFE3-L protein consisting of 327 amino acids encoded by nucleotides 361-2469 of the cloned cDNA.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 50 kDa

Comments:

Related Products: ABIN967389, ABIN968553

Application Details

Application Notes: Applications include western blot analysis (0.25-1.0 µg/ml). WI-38 cells are suggested as a positive control. Other applications include gel shift (supershifts) and immunoprecipitation, which are not routinely tested. The antibody has been used to detect in vitro translated TFE3-L and TFE3-L, expressed as a recombinant protein in bacteria. In gel shift assays, using µE3 as a probe, this antibody supershifts the complex.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors, Adaptive Immunity

Restrictions: For Research Use only

Images

Western blot analysis of TFE3. Lysate from WI-38 cells was probed with anti- TFE3 (clone G138-312) at concentrations of 1.0 (lane 1), 0.5 (lane 2), and 0.25 myg/ml (lane 3). TFE3 is identified as a band of 50 kDa.

anti-Transcription Factor E3 (TFE3) antibody (Image 2)

Publications

Fisher, Parent, Sharp: "Myc/Max and other helix-loop-helix/leucine zipper proteins bend DNA toward the minor groove." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 24, pp. 11779-83, 1993 (PubMed).

Roman, Matera, Cooper et al.: "mTFE3, an X-linked transcriptional activator containing basic helix-loop-helix and zipper domains, utilizes the zipper to stabilize both DNA binding and multimerization." in: Molecular and cellular biology, Vol. 12, Issue 2, pp. 817-27, 1992 (PubMed).

Beckmann, Su, Kadesch: "TFE3: a helix-loop-helix protein that activates transcription through the immunoglobulin enhancer muE3 motif." in: Genes & development, Vol. 4, Issue 2, pp. 167-79, 1990 (PubMed).

Murre, McCaw, Baltimore: "A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins." in: Cell, Vol. 56, Issue 5, pp. 777-83, 1989 (PubMed).

Zhao, Zhao, Zhou et al.: "TFEC, a basic helix-loop-helix protein, forms heterodimers with TFE3 and inhibits TFE3-dependent transcription activation." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4505-12, 1993 (PubMed).