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Myogenin antibody (Myogenin (Myogenic Factor 4)) (AA 30-224)

Details for Product anti-MYOG Antibody No. ABIN967512, Supplier: Login to see New
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Synonyms cb553, zgc:100763, LOC446037, myf4, Xmyog, myogenin, LOC100136471, LOC100224062, LOC100303673, MYF4, bHLHc3, myo, myf-4
AA 30-224
(48), (23), (19), (16), (15), (15), (12), (11), (9), (5), (5), (5), (4), (2), (2), (2), (2), (1), (1), (1)
Cat (Feline), Human, Mouse (Murine), Rat (Rattus)
(119), (64), (48), (14), (13), (8), (4), (1), (1), (1)
(78), (74), (14)
Clonality (Clone)
Monoclonal ()
This Myogenin antibody is un-conjugated
(8), (8), (8), (8), (8), (8), (1), (1)
BioImaging (BI), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Intracellular Staining (ICS), Western Blotting (WB)
(133), (83), (24), (24), (22), (19), (11), (9), (9), (5), (4), (3), (2), (1), (1), (1), (1)
Pubmed 6 references available
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Quantity 0.1 mg
Shipping to United States ( )
Brand BD Pharmingen™
Immunogen Rat Myogenin aa. 30-224 Recombinant Protein
Clone F5D
Isotype IgG1
Cross-Reactivity Human, Mouse (Murine), Cat (Feline)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Myogenin (MYOG Antibody Abstract)
Background Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculin or Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. In addition to activating muscle-specific genes, members of the MyoD family members activate their own transcription and transactivate the transcription of other MyoD family members. For example, transfection of myogenin into 10T1/2 cells or Swiss 3T3 cells results in the activation of the endogenous myogenin gene as well as transactivation of MyoD. Likewise, the transfection of MyoD into these cells results in the activation of MyoD as well as the transactivation of myogenin. Each member of the MyoD family has distinct roles in muscle development, myogenin plays a key role in muscle maturation. Myogenin migrates at a molecular weight of ~34 kDa by SDS-PAGE. Clone F5D recognizes human, mouse, rat and cat myogenin. Mapping studies suggest that F5D reacts with an epitope between amino acids 138-158 of myogenin. A recombinant fusion protein corresponding to amino acids 30- 224 of rat myogenin was used as immunogen.
Molecular Weight 34 kDa
Research Area Stem Cells, Transcription Factors, Cytoskeleton, Cell/Tissue Markers
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-Myogenin antibody (Myogenin (Myogenic Factor 4)) (AA 30-224) (ABIN967512) Western blot analysis of Myogenin. Lysates from Rh30 rhabdomyosarcoma cells were prob...
 image for anti-Myogenin antibody (Myogenin (Myogenic Factor 4)) (AA 30-224) (ABIN967512) anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 30-224) antibody (Image 2)
Product cited in: Ryan, Liu, Chu et al.: "Retinoic acid enhances skeletal myogenesis in human embryonic stem cells by expanding the premyogenic progenitor population." in: Stem cell reviews, Vol. 8, Issue 2, pp. 482-93, 2012 (PubMed).

Meadows, Cho, Flynn et al.: "Myogenin regulates a distinct genetic program in adult muscle stem cells." in: Developmental biology, Vol. 322, Issue 2, pp. 406-14, 2009 (PubMed).

Gang, Jeong, Hong et al.: "Skeletal myogenic differentiation of mesenchymal stem cells isolated from human umbilical cord blood." in: Stem cells (Dayton, Ohio), Vol. 22, Issue 4, pp. 617-24, 2004 (PubMed).

Dias, Dilling, Houghton: "The molecular basis of skeletal muscle differentiation." in: Seminars in diagnostic pathology, Vol. 11, Issue 1, pp. 3-14, 1994 (PubMed).

Wang, Marx, McNutt et al.: "Expression of myogenic regulatory proteins (myogenin and MyoD1) in small blue round cell tumors of childhood." in: The American journal of pathology, Vol. 147, Issue 6, pp. 1799-810, 1996 (PubMed).

Background publications Miyashita, Krajewski, Krajewska et al.: "Tumor suppressor p53 is a regulator of bcl-2 and bax gene expression in vitro and in vivo." in: Oncogene, Vol. 9, Issue 6, pp. 1799-805, 1994 (PubMed).

Catalog No. ABIN967512
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