Myogenin (Myogenic Factor 4) (MYOG) (AA 30-224) antibody

Antigen: Myogenin (Myogenic Factor 4) (MYOG)

Synonyms: myo, MYF4, bHLHc3, cb553, zgc:100763, MYOG, myogenin, LOC446037, myog, myf4, Xmyog, LOC100136471, LOC100224062, LOC100303673

Binding Site: AA 30-224

Clonality: Monoclonal (F5D)

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN967512

Additional Information

Alternative name: Myogenin

Immunogen: Recombinant Rat Myogenin

Cross-Reactivity: Human, Mouse (Murine), Cat (Feline)

Format: Liquid

Isotype: IgG1

Clone: F5D

Description: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculin or Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. In addition to activating muscle-specific genes, members of the MyoD family members activate their own transcription and transactivate the transcription of other MyoD family members. For example, transfection of myogenin into 10T1/2 cells or Swiss 3T3 cells results in the activation of the endogenous myogenin gene as well as transactivation of MyoD. Likewise, the transfection of MyoD into these cells results in the activation of MyoD as well as the transactivation of myogenin. Each member of the MyoD family has distinct roles in muscle development, myogenin plays a key role in muscle maturation. Myogenin migrates at a molecular weight of ~34 kDa by SDS-PAGE. Clone F5D recognizes human, mouse, rat and cat myogenin. Mapping studies suggest that F5D reacts with an epitope between amino acids 138-158 of myogenin. A recombinant fusion protein corresponding to amino acids 30- 224 of rat myogenin was used as immunogen.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Stem Cells, Transcription Factors, Cytoskeleton, Cell/Tissue Markers

Restrictions: For Research Use only

Images

Western blot analysis of Myogenin. Lysates from Rh30 rhabdomyosarcoma cells were probed with anti-Myogenin antibody (clone FSD) at 2 myg/ml.

anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 30-224) antibody (Image 2)

Publications

Product: Miyashita, Krajewski, Krajewska et al.: "Tumor suppressor p53 is a regulator of bcl-2 and bax gene expression in vitro and in vivo." in: Oncogene, Vol. 9, Issue 6, pp. 1799-805, 1994 (PubMed).

Dias, Dilling, Houghton: "The molecular basis of skeletal muscle differentiation." in: Seminars in diagnostic pathology, Vol. 11, Issue 1, pp. 3-14, 1994 (PubMed).