Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody

Antigen: Fas Ligand (TNF Superfamily, Member 6) (FASL)

Synonyms: FASL, CD178, CD95L, TNFSF6, APT1LG1, gld, Fas-L, Faslg, Tnfsf6, Fas-Ligand, FASLG, fasl, APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6, lpr, APO1, TNFR6, Tnfrsf6, AI196731, Fasl, CD95-L, Apt1Lg1, FAS, LOC574159, zgc:162027

Clonality: Monoclonal (G247-4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Catalog no.: ABIN967520

Additional Information

Alternative name: CD178

Immunogen: Recombinant Human FasL

Format: Liquid

Isotype: IgG1

Clone: G247-4

Description: Fas (APO-1, CD95) is a 45 kD cell surface protein that mediates apoptosis when crosslinked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary and lung. FasL is a member of the TNF cytokine family that induces apoptosis by binding to Fas, its cell-surface receptor. FasL may exist in both membrane and soluble forms and expressed on activated T cells, NK cells, and other immunologically privileged” sites. Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development.
G247-4 recognizes human FasL. It recognizes both the membrane bound (FasL) and soluble (sFasL) forms. A recombinant protein containing the external domain of human FasL was used as immunogen. FasL and sFasL migrate at reduced molecular weights of 40 and 26 kD, respectively. However, the molecular weights observed in a particular sample may vary according to FasL and sFasL glycosylation and breakdown patterns as described in Tanaka et al. For example, FasL may migrate as a doublet of 40 and 42 kD.
Synonyms: Fas Ligand, CD95 Ligand

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 42 kDa, 40 kDa (membrane), 26 kDa (soluble)

Comments:

Related Products: ABIN967389

Synonyms: FASL, CD178, CD95L, TNFSF6, APT1LG1, gld, Fas-L, Faslg, Tnfsf6, Fas-Ligand, FASLG, fasl, APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6, lpr, APO1, TNFR6, Tnfrsf6, AI196731, Fasl, CD95-L, Apt1Lg1, FAS, LOC574159, zgc:162027

Application Details

Application Notes: Applications include immunoprecipitation (1-2 µg/ml), western blot analysis (1-2 µg/ml) and immunohistochemical staining of acetone-fixed frozen tissue sections (0.5-4 µg/ml). G247-4 is not recommended for flow cytometry. For flow cytometry application, clone NOK-1 (purified, or biotin-conjugated) is recommended.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Restrictions: For Research Use only

Images

Western blot analysis of Fas Ligand (FasL). L5187Y human T lymphoma cells were transfected with human FasL cDNA and then not treated (lane 1) or treated (lane 2) with the metalloproteinase inhibitor, KB8301. KB8301 blocks FasL cleavage from the cell surface, resulting in high levels membrane expressed FasL. The blot shows that whereas FasL is detected in untreated cells (lane 1), levels increased dramatically when cells were treated with KB8301 (lane 2). A mouse IgG1 isotype matched antibody was used as a negative control (lane 3).

anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (Image 2)

Publications

Product: Kayagaki, Kawasaki, Ebata et al.: "Metalloproteinase-mediated release of human Fas ligand." in: The Journal of experimental medicine, Vol. 182, Issue 6, pp. 1777-83, 1996 (PubMed).

Takahashi, Tanaka, Brannan et al.: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, 1994 (PubMed).

Tanaka, Suda, Takahashi et al.: "Expression of the functional soluble form of human fas ligand in activated lymphocytes." in: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, 1995 (PubMed).

Griffith, Ferguson: "The role of FasL-induced apoptosis in immune privilege." in: Immunology today, Vol. 18, Issue 5, pp. 240-4, 1997 (PubMed).

Sträter, Wellisch, Riedl et al.: "CD95 (APO-1/Fas)-mediated apoptosis in colon epithelial cells: a possible role in ulcerative colitis." in: Gastroenterology, Vol. 113, Issue 1, pp. 160-7, 1997 (PubMed).

Orlinick, Vaishnaw, Elkon et al.: "Requirement of cysteine-rich repeats of the Fas receptor for binding by the Fas ligand." in: The Journal of biological chemistry, Vol. 272, Issue 46, pp. 28889-94, 1997 (PubMed).

Orlinick, Elkon, Chao: "Separate domains of the human fas ligand dictate self-association and receptor binding." in: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32221-9, 1998 (PubMed).