PI3 Kinase P85 alpha antibody

Details for Product No. ABIN967523
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Antigen
Synonyms AA414921, C530050K14, PI3K, p50alpha, p55alpha, p85alpha, AGM7, GRB1, p85, p85-ALPHA, PI3KA
Reactivity
Cow (Bovine)
(148), (100), (85), (42), (36), (36), (12), (2), (1), (1)
Host
Mouse
(136), (30)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB), Immunoprecipitation (IP), ELISA
(125), (61), (44), (30), (30), (18), (11), (10), (8), (3), (3), (2), (2), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN967523
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Immunogen Recombinant cow p85alpha
Clone U15
Isotype IgG1
Cross-Reactivity Human, Monkey
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name pi 3-Kinase p85 alpha
Background Phosphatidylinositol 3-kinase (PI3-kinase) is a universal enzyme associated with receptor signaling pathways. It generates second messenger phospholipids by phosphorylating the D-3 position of inositol phospholipids including phosphatidylinositol (PI), PI-4-phosphate, and PI-4, 5-biphosphate. The enzyme exists as a heterodimer composed of regulatory 85 kDa (p85) and catalytic 110 kDa (p110) subunits. The p85 subunit contains two SH2 (src-homology 2) domains and an SH3 domain. The catalytic activity of the p110 subunit is stimulated when the p85 regulatory subunit binds, through its SH2 domains, to activated receptor and non-receptor tyrosine kinases. Two p85 isoforms have been described, p85a and p85ß. Both isoforms bind to activated receptors and each may be responsible for mediating a subset of PI3-kinase interactions. Clone U15 recognizes the p85 regulatory subunit of PI3 kinase (p85alpha). It reacts with human, monkey, and cow PI3 kinase. It does not cross-react with mouse or rat PI3 kinase. Recombinant cow p85alpha was used as immunogen. The epitope has been mapped to the inter-SH2 spacer region of p85alpha. The antibody will block lipids binding to this region.
Molecular Weight 85 kDa
Application Notes Clone U15 was originally characterized by ELISA, immunoprecipitation, and western blot analysis. Applications include immunoprecipitation (1-2 µg/1x10^6 cells) and western blot analysis (1-2 µg/ml). In immunoprecipitation, U15 can bring down an active kinase. Jurkat human T cells (ATCC TIB-152) are suggested as a positive control.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-PI3 Kinase P85 alpha antibody Western blot analysis of the p85 regulatory subunit of PI3 kinase (p85alpha). Lysates from Jurkat human T cells were probed with anti-PI3 Kinase (clone U15, ABIN967523). The antibody identifies p85alpha as an 85 kDa band.
anti-PI3 Kinase P85 alpha antibody (2) anti-PI3 Kinase P85 alpha antibody (Image 2)
Product cited in: Whitman, Downes, Keeler et al.: "Type I phosphatidylinositol kinase makes a novel inositol phospholipid, phosphatidylinositol-3-phosphate." in: Nature, Vol. 332, Issue 6165, pp. 644-6, 1988 (PubMed).

Shoelson, Sivaraja, Williams et al.: "Specific phosphopeptide binding regulates a conformational change in the PI 3-kinase SH2 domain associated with enzyme activation." in: The EMBO journal, Vol. 12, Issue 2, pp. 795-802, 1993 (PubMed).

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