Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1) antibody

Details for Product No. ABIN967560
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Synonyms ASCL1, ASH1, Mash1, Xash1, ascl1-A, HASH1, MASH1, bHLHa46, AI225900, ASH1L1, KMT2H
Rat (Rattus)
(113), (34), (23), (2), (2), (1), (1), (1), (1)
(103), (12), (3), (2)
Clonality (Clone)
Monoclonal ()
(9), (9), (9), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Immunocytochemistry (ICC)
(106), (73), (36), (12), (10), (8), (2), (2), (2), (2), (1), (1), (1)
Pubmed 6 references available
Quantity 0.1 mg
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Catalog No. ABIN967560
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Immunogen Rat MASH1 full length recombinant protein
Clone 24B72D11-1
Isotype IgG1
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name MASH1
Background MASH1 and MASH2 (Mammalian achaete-schute Homolog 1 and 2) are basic helixloop-helix transcription factors which are mammalian homologs of the achaete-schute gene family that is required for neuronal development in Drosophila. The bHLH motif is present in many types of transcription factors including E2-2, E47, MyoD and others. MASH1 has been shown to heterodimerize with other bHLH containing transcription factors. MASH1-E12 heteromers bind to and promote transcriptional activation of muscle creatine kinase, a known target for MyoD activation. However, MASH1 appears to play its primary role during early development of the autonomic nervous system. In committed neuronal precursor cells, early expression of MASH1 activates a subset of neuron-specific genes to promote differentiation. Thus null mutations in the MASH1 gene suggest that MASH1 is a valuable marker for investigation of neural development in mammals. MASH1 is observed as an ~34 kDa protein by SDS-PAGE.
Molecular Weight 34 kDa
Application Notes The 24B72D11.1 antibody is recommended for western blot analysis (0.5-2.0 µg/ml). Rat embryonic brain can be used as a positive control. Neuro2A mouse neuroblastoma cells (ATCC CCL-131) may also be used as a positive control for this application.

The 24B72D11.1 antibody is reported in the literature to work well for immunohistochemistry.

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1) antibody Western blot analysis of MASH1. Lysate from rat embryonic brain was probed with anti-MASH1 (clone 24B72D11.1, ABIN967560) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). MASH1 is identified as a band of ~34 kDa.
anti-Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1) antibody (2) anti-Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1) antibody (Image 2)
Product cited in: Ivaniutsin, Chen, Mason et al.: "Adenomatous polyposis coli is required for early events in the normal growth and differentiation of the developing cerebral cortex." in: Neural development, Vol. 4, Issue 1, pp. 3, 2009 (PubMed).

Ralston, Chiriboga, Nonaka: "MASH1: a useful marker in differentiating pulmonary small cell carcinoma from Merkel cell carcinoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 21, Issue 11, pp. 1357-62, 2008 (PubMed).

Hu, Ippolito, Garabedian et al.: "Molecular characterization of a metastatic neuroendocrine cell cancer arising in the prostates of transgenic mice." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44462-74, 2002 (PubMed).

Sommer, Shah, Rao et al.: "The cellular function of MASH1 in autonomic neurogenesis." in: Neuron, Vol. 15, Issue 6, pp. 1245-58, 1996 (PubMed).

Lo, Johnson, Wuenschell et al.: "Mammalian achaete-scute homolog 1 is transiently expressed by spatially restricted subsets of early neuroepithelial and neural crest cells." in: Genes & development, Vol. 5, Issue 9, pp. 1524-37, 1991 (PubMed).

Johnson, Birren, Saito et al.: "DNA binding and transcriptional regulatory activity of mammalian achaete-scute homologous (MASH) proteins revealed by interaction with a muscle-specific enhancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 8, pp. 3596-600, 1992 (PubMed).

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