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P-Glycoprotein antibody

Details for Product No. ABIN967562, Supplier: Log in to see
Antigen
  • Abcb1
  • Mdr1a
  • MDR1
  • ABCB1
  • ABCB4
  • CMDR1
  • xemdr
Reactivity
Human
23
4
4
2
1
Host
Mouse
16
8
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
18
8
7
5
4
4
2
1
1
Supplier
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Brand BD Pharmingen™
Clone 17F9
Isotype IgG2b
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Background Reacts with a 170-180 kDa transmembrane glycoprotein (P-glycoprotein), a product of the multidrug resistance-1 (MDR1) gene. This glycoprotein is expressed on MDR positive cells and has been reported to be expressed on many normal tissues, such as adrenal glands and endothelium, in the brain and skin. P-glycoprotein is known to impart drug resistance to cells by pumping many anti-cancer drugs out of the cytoplasm. 17F9 antibody binds to the surface of the viable, unfixed MDR+ cell line, PMG-Y (please inquire), and is able to partially block the binding of UIC2 antibody (another MDR-specific monoclonal antibody). Immunoprecipitation application is reported, but not routinely tested in house.
Synonyms: MDR
Pathways
Application Notes Positive control cells are available through special order
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Flow Cytometry (FACS) image for anti-P-Glycoprotein antibody (ABIN967562) Profile of P-glycoprotein (MDR) expressed on PMG-Y cell line analyzed on a FACScan (B...
Product cited in: Shi, Wrin, Reeder et al.: "High-affinity monoclonal antibodies against P-glycoprotein." in: Clinical immunology and immunopathology, Vol. 76, Issue 1 Pt 1, pp. 44-51, 1995 (PubMed).

Goldstein, Galski, Fojo et al.: "Expression of a multidrug resistance gene in human cancers." in: Journal of the National Cancer Institute, Vol. 81, Issue 2, pp. 116-24, 1989 (PubMed).

Bénard, Bourhis, Riou: "Clinical significance of multiple drug resistance in human cancers." in: Anticancer research, Vol. 10, Issue 5A, pp. 1297-302, 1990 (PubMed).