Images for product: anti-Ezrin (EZR) (pThr567) antibody
Western blot analysis of Ezrin (pT567) in human epidermis. Lysates from control (lanes 1-3) and human epidermal growth factor-treated (lanes 4-6) human A-431 epidermoid carcinoma (ABIN968533 and ABIN968534, respectively) were probed with purified mAb J37-954.281.307 at concentrations of 0.03125 (lanes 1 and 4), 0.0156 (lanes 2 and 5), and 0.0078 µg/ml (lanes 3 and 6). The bands appear as a doublet with Ezrin (pT567) and Radixin (pT564) identifiable at 80 kD and Moesin (pT558) identifiable at 75 kD in the growth factor treated cells.
Ezrin (pT567) staining on human breast cancer. Following antigen retrieval with BD Retrievagen A buffer, the formalin-fixed paraffin-embedded sections were either left untreated (left column) or treated with a phosphatase to eliminate all phosphorylation (right column). The tissue sections were stained with either purified Mouse anti-Ezrin (ABIN968057 or ABIN968058, top row) or purified Mouse anti-Ezrin (pT567) (bottom row) with Hematoxylin counterstaining. Original magnification: 40X.