Ezrin (EZR) (pThr567) antibody

Details for Product No. ABIN967590
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Synonyms ezr, vil2, wu:fc05c03, wu:fj31d06, cvil, cvl, CVIL, CVL, VIL2, AW146364, R75297, Vil2, p81, ATVLN2, T11A7.16, T11A7_16, villin 2
(35), (26), (25), (23), (18), (17), (13), (12), (8), (8), (8), (6), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(332), (196), (168), (5), (2), (2), (2), (1)
(278), (28), (25), (2), (1)
Clonality (Clone)
Monoclonal ()
(10), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1)
Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(283), (127), (62), (52), (50), (19), (12), (8), (7), (7), (5), (4), (4), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN967590
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Immunogen Phosphorylated Ezrin Peptide
Clone J37-954-281-307
Isotype IgG1, kappa
Cross-Reactivity (Details) Predicted: Mouse, Rat
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Ezrin
Background Ezrin is a member of the ERM (Ezrin-Radixin-Moesin) family of proteins that can function as a crosslinker between the actin cytoskeleton and the plasma membrane of the cell. Phosphorylation of threonine residues (Thr567 on Ezrin, Thr564 on Radixin and Thr558 on Moesin) has been reported to occur with stimulation of growth factors and is important for cytoskeletal rearrangements. Phosphorylation of Thr567 at the C-terminal F actin-binding domain has been reported to activate the conversion of Ezrin from a dormant soluble form in the cytosol to a membrane- and actin-binding conformation.
In addition to its reactivity to the human species, the peptide used to generate this antibody is either identical or has homology to analogous regions of mouse and rat Ezrin, Radixin and Moesin. The J37-954.281.307 monoclonal antibody has been reported to recognize phosphorylated T567 on active Ezrin in addition to crossreacting on phosphorylated T564 on Radixin and phosphorylated T558 on Moesin.
Molecular Weight 75-80 kDa
Research Area Cancer, Cytoskeleton, Microfilaments

Related Products: ABIN967389, ABIN968533, ABIN968534, ABIN968057

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Ezrin (EZR) (pThr567) antibody Western blot analysis of Ezrin (pT567) in human epidermis. Lysates from control (lanes 1-3) and human epidermal growth factor-treated (lanes 4-6) human A-431 epidermoid carcinoma (ABIN968533 and ABIN968534, respectively) were probed with purified mAb J37-954.281.307 at concentrations of 0.03125 (lanes 1 and 4), 0.0156 (lanes 2 and 5), and 0.0078 µg/ml (lanes 3 and 6). The bands appear as a doublet with Ezrin (pT567) and Radixin (pT564) identifiable at 80 kD and Moesin (pT558) identifiable at 75 kD in the growth factor treated cells.
anti-Ezrin (EZR) (pThr567) antibody (2) Ezrin (pT567) staining on human breast cancer. Following antigen retrieval with BD Retrievagen A buffer, the formalin-fixed paraffin-embedded sections were either left untreated (left column) or treated with a phosphatase to eliminate all phosphorylation (right column). The tissue sections were stained with either purified Mouse anti-Ezrin (ABIN968057 or ABIN968058, top row) or purified Mouse anti-Ezrin (pT567) (bottom row) with Hematoxylin counterstaining. Original magnification: 40X.
Product cited in: Tran Quang, Gautreau, Arpin et al.: "Ezrin function is required for ROCK-mediated fibroblast transformation by the Net and Dbl oncogenes." in: The EMBO journal, Vol. 19, Issue 17, pp. 4565-76, 2000 (PubMed).

Gautreau, Louvard, Arpin: "Morphogenic effects of ezrin require a phosphorylation-induced transition from oligomers to monomers at the plasma membrane." in: The Journal of cell biology, Vol. 150, Issue 1, pp. 193-203, 2000 (PubMed).

Gautreau, Poullet, Louvard et al.: "Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 13, pp. 7300-5, 1999 (PubMed).

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