You are viewing an incomplete version of our website. Please click to reload the website as full version.

NFKB1 antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (pSer529)

Details for Product anti-NFKB1 Antibody No. ABIN967610, Supplier: Login to see
Antigen
  • ebp-1
  • kbf1
  • nf-kappa-b
  • nf-kappaB
  • nfkb-p105
  • nfkb-p50
  • NF-KB1
  • NF-kappaB
  • NF-kappaB1
  • p105
  • p50
  • p50/p105
  • EBP-1
  • KBF1
  • NF-kB1
  • NF-kappa-B
  • NFKB-p105
  • NFKB-p50
  • NFkappaB
  • BICDR-1
  • bicdr1
  • ccdc64
  • NF-kB
Alternatives
anti-Human NFKB1 antibody for Western Blotting
Epitope
pSer529
46
43
43
39
37
36
30
13
13
13
11
10
8
7
7
7
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
512
151
133
16
16
13
11
1
1
Host
Mouse
490
44
4
3
Clonality (Clone)
Monoclonal ()
Conjugate
This NFKB1 antibody is un-conjugated
17
15
12
8
6
6
6
6
6
6
6
6
4
4
4
Application
Intracellular Staining (ICS), Western Blotting (WB)
404
218
162
103
97
70
47
24
15
10
9
9
4
4
3
2
1
1
Supplier
Login to see
Supplier Product No.
Login to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Brand BD Pharmingen™
Immunogen Phosphorylated Human NF-kappaB p65 Peptide
Clone K10-895-12-50
Isotype IgG2b, kappa
Characteristics 1. Please refer to us for technical protocols.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name NF-kappa B (NFKB1 Antibody Abstract)
Background Nuclear factor kB (NF-kB) is a ubiquitously expressed transcription factor that regulates the expression of 200-300 genes. It is crucial for basic cellular responses to stress and pathogens, such as proliferation, survival, development, and apoptosis. The most studied NF-kB complex consists of the p50 (also known as NF-kB1) and p65 (also known as REL-A) subunits, both containing a 300-amino acid region with homology to the Rel proto-oncogene product (RH domain). The RH domain contains motifs for dimerization, nuclear localization, and binding to specific DNA sequences. In addition to the RH domain, the p65 subunit contains the transactivation domain, which is responsible for the interaction with the inhibitor IkB and which contains phosphorylation sites. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IkB. This complex prevents nuclear translocation and activity of NF-kB. In response to stimuli such as cytokines, LPS, DNA damage, and viral infections, IkB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IkB/NF-kB complex, allowing the free heterodimeric NF-kB to translocate to the nucleus. Furthermore, optimal activation of NF-kB requires phosphorylation in the transactivation domain of p65. In the nucleus, activated NF-kB dimers bind to the kB sites within promoters and enhancers and function as transcriptional activators. The K10-895.12.50 monoclonal antibody recognizes the phosphorylated serine 529 (pS529) in the transactivation domain of human NF-kB p65 subunit.
Molecular Weight 65 kDa
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-NFKB1 antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (pSer529) (ABIN967610) Western blot analysis of NF-kappaB p65 (pS529) in transformed human epithelioid carci...
Western Blotting (WB) image for anti-NFKB1 antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (pSer529) (ABIN967610) Western blot analysis of NF-kappaB p65 (pS529) in human peripheral blood mononuclear ...
 image for anti-NFKB1 antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (pSer529) (ABIN967610) anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (pSer529) antibody (Image 3)
Product cited in: Siebenlist, Brown, Claudio: "Control of lymphocyte development by nuclear factor-kappaB." in: Nature reviews. Immunology, Vol. 5, Issue 6, pp. 435-45, 2005 (PubMed).

Natoli, Saccani, Bosisio et al.: "Interactions of NF-kappaB with chromatin: the art of being at the right place at the right time." in: Nature immunology, Vol. 6, Issue 5, pp. 439-45, 2005 (PubMed).

Viatour, Merville, Bours et al.: "Phosphorylation of NF-kappaB and IkappaB proteins: implications in cancer and inflammation." in: Trends in biochemical sciences, Vol. 30, Issue 1, pp. 43-52, 2005 (PubMed).

Did you look for something else?