CD247 Molecule (CD247) (pTyr142) antibody

Antigen: CD247 Molecule (CD247)

Synonyms: T3Z, CD3H, CD3Q, CD3Z, TCRZ, CD3-ZETA, Cd3, T3z, Cd3h, Cd3z, TCRk, Tcrz, CD3-eta, CD3zeta, AW552088, CD3-zeta, 4930549J05Rik, A430104F18Rik, CD247, im:7156386, LOC100136514, LOC100136549, LOC100305023

Binding Site: pTyr142

Clonality: Monoclonal (K25-407-69)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN967618

Additional Information

Alternative name: CD247 (CD3z)

Immunogen: Phosphorylated Human CD3zeta Peptide

Format: Liquid

Isotype: IgG2a, kappa

Clone: K25-407-69

Description: The T cell receptor (TCR), expressed by thymocytes and T lymphocytes, is a multi-component cell-surface complex responsible for recognizing antigen in the context of MHC molecules. The antigen-specific binding component of the TCR, Ti, is a heterodimer of the variable Ig-like subunits a and b or g and d. Ti is non-covalently associated with an invariant set of molecules referred to as the CD3 polypeptides, g, d, e, and zeta. The CD3 z polypeptide (CD3z) was named CD247 at the 7th Human Leukocyte Differentiation Antigens Workshop. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. After antigen recognition by the TCR, CD3z is the primary intracellular signal transducing subunit. It contains three ITAMs (Immunoreceptor Tyrosine-based Activation Motifs), each of which contains a pair of tyrosine residues that are phosphorylated by Lck and Fyn and are required for signal propagation. The molecular weight of CD3z is 16 kDa, and it is also observed as 32-kDa homodimers or as heterodimers with the g chain of Fc receptors. Upon phosphorylation, the CD3z monomer undergoes an apparent shift in electrophoretic mobility up to 21 kDa. The K25-407.69 monoclonal antibody recognizes the phosphorylated tyrosine 142 (pY142) in the third ITAM domain of human CD3z (CD247).
Synonyms: CD3zeta

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 21 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Restrictions: For Research Use only

Images

Western blot analysis of CD3z (CD247) (pY142) in human T lymphocytes. Lysates from control (lanes 1-3) and anti-CD3- plus anti-CD28-activated (lanes 4-6) Jurkat cells were probed with purified mouse anti-CD3z (CD247) (pY142) at concentrations of 0.5 (anes 1 and 4), 0.25 (lanes 2 and 5), and 0.125 µg/ml (lanes 3 and 6). CD3z (CD247) (pY142) is identified as a band of 21 kDa in the treated cells.

Publications

Product: Alberola-Ila, Takaki, Kerner et al.: "Differential signaling by lymphocyte antigen receptors." in: Annual review of immunology, Vol. 15, pp. 125-54, 1997 (PubMed).

Kersh, Shaw, Allen: "Fidelity of T cell activation through multistep T cell receptor zeta phosphorylation." in: Science (New York, N.Y.), Vol. 281, Issue 5376, pp. 572-5, 1998 (PubMed).

Ernst, Shih: "CD3 complex." in: Journal of biological regulators and homeostatic agents, Vol. 14, Issue 3, pp. 226-9, 2001 (PubMed).

Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).