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CD247 antibody (pTyr142)

CD247 Reactivity: Human WB Host: Mouse Monoclonal K25-407-69 unconjugated
Catalog No. ABIN967618
  • Target See all CD247 Antibodies
    CD247 (CD247 Molecule (CD247))
    Binding Specificity
    • 13
    • 13
    • 8
    • 8
    • 7
    • 6
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr142
    Reactivity
    • 94
    • 42
    • 18
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 75
    • 16
    • 6
    • 1
    Mouse
    Clonality
    • 73
    • 25
    Monoclonal
    Conjugate
    • 56
    • 9
    • 8
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This CD247 antibody is un-conjugated
    Application
    • 73
    • 53
    • 35
    • 14
    • 11
    • 10
    • 7
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Brand
    BD Pharmingen™
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Phosphorylated Human CD3zeta Peptide
    Clone
    K25-407-69
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product CD247 Primary Antibody
  • Comment

    Related Products: ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Salomon, Ficarro, Brill, Brinker, Phung, Ericson, Sauer, Brock, Horn, Schultz, Peters: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, (2003) (PubMed).

    Ernst, Shih: "CD3 complex." in: Journal of biological regulators and homeostatic agents, Vol. 14, Issue 3, pp. 226-9, (2001) (PubMed).

    Kersh, Shaw, Allen: "Fidelity of T cell activation through multistep T cell receptor zeta phosphorylation." in: Science (New York, N.Y.), Vol. 281, Issue 5376, pp. 572-5, (1998) (PubMed).

    Alberola-Ila, Takaki, Kerner, Perlmutter: "Differential signaling by lymphocyte antigen receptors." in: Annual review of immunology, Vol. 15, pp. 125-54, (1997) (PubMed).

  • Target
    CD247 (CD247 Molecule (CD247))
    Alternative Name
    CD247 (CD3z) (CD247 Products)
    Synonyms
    CD3-ZETA antibody, CD3H antibody, CD3Q antibody, CD3Z antibody, T3Z antibody, TCRZ antibody, 4930549J05Rik antibody, A430104F18Rik antibody, AW552088 antibody, Cd3 antibody, Cd3-eta antibody, Cd3-zeta antibody, Cd3h antibody, Cd3z antibody, Cd3zeta antibody, T3z antibody, Tcrk antibody, Tcrz antibody, LOC100136514 antibody, LOC100136549 antibody, LOC100305023 antibody, CD247 antibody, TCRzeta antibody, CD247 molecule antibody, CD247 antigen antibody, CD3zeta-2 antibody, CD3zeta-1 antibody, Cd247 molecule antibody, CD247 antibody, Cd247 antibody, cd3z antibody, cd247 antibody
    Background
    The T cell receptor (TCR), expressed by thymocytes and T lymphocytes, is a multi-component cell-surface complex responsible for recognizing antigen in the context of MHC molecules. The antigen-specific binding component of the TCR, Ti, is a heterodimer of the variable Ig-like subunits a and b or g and d. Ti is non-covalently associated with an invariant set of molecules referred to as the CD3 polypeptides, g, d, e, and zeta. The CD3 z polypeptide (CD3z) was named CD247 at the 7th Human Leukocyte Differentiation Antigens Workshop. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. After antigen recognition by the TCR, CD3z is the primary intracellular signal transducing subunit. It contains three ITAMs (Immunoreceptor Tyrosine-based Activation Motifs), each of which contains a pair of tyrosine residues that are phosphorylated by Lck and Fyn and are required for signal propagation. The molecular weight of CD3z is 16 kDa, and it is also observed as 32-kDa homodimers or as heterodimers with the g chain of Fc receptors. Upon phosphorylation, the CD3z monomer undergoes an apparent shift in electrophoretic mobility up to 21 kDa. The K25-407.69 monoclonal antibody recognizes the phosphorylated tyrosine 142 (pY142) in the third ITAM domain of human CD3z (CD247).
    Synonyms: CD3zeta
    Molecular Weight
    21 kDa
    Pathways
    TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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