TNF Receptor-Associated Factor 2 (TRAF2) (AA 93-199) antibody

Antigen: TNF Receptor-Associated Factor 2 (TRAF2)

Synonyms: TRAP, TRAP3, MGC:45012, LAP1, CAP-1, CRAF1, CD40bp, RNF85, MGC:3310, AI325259, amn, T-BAM, AI528849, AI851288, 2310003F17Rik, C630032O20Rik, MGC83471, TANK, TRAF2, LOC716907, LOC100218184, Traf2

Binding Site: AA 93-199

Clonality: Monoclonal (C90-481)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN967631

Additional Information

Alternative name: TRAF2

Immunogen: Human TRAF2

Format: Liquid

Isotype: IgG2a

Clone: C90-481

Description: TRAFs (TNF receptor associated factors) are signal transducing molecules which participate in forming TNF/Nerve Growth Factor receptor-associated signalling complexes. TRAF proteins share a novel C-terminal homology region, the TRAF” domain, a coiled-coiled region and the majority have Zinc/RING finger motifs. At least six TRAF proteins (TRAFs 1-6) have been identified. These proteins are cytoplasmic adapters, they bind to cytoplasmic domains of various receptors and can function to recruit other proteins to a signaling complex thereby promoting intracellular signal transduction. Specifically, TRAF2 has been shown to interact with the following surface receptors: TNFRII, CD27, CD30, CD40, 4-1BB, Ox40, HVEM/ATAR and LMP-1. TRAF2 has also been shown to associate with intracellular proteins, including TRADD, FADD, I-TRAF/TANK, TRIP, A20, c-IAP1 and 2, Casper, RIP and NIK. Thus, the interaction of TRAF2 with these various receptors and intracellular proteins plays an important role modulating downstream events which can result in inducing cell death or keeping cells alive. For example, cells deficient in TRAF2 demonstrated increased sensitivity to TNF-induced cell death, but a decrease in TNF-induced JNK activation, and little change on NF-kB activation. TRAF2 is detected at ~ 53 kDa in western blot analysis. The C90-481 antibody reacts with human TRAF2. A synthetic protein freagment corresponding to amino acids 93-199 of human TRAF2 was used as immunogen.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 53 kDa

Comments:

Related Products: ABIN967389, ABIN968537

Application Details

Application Notes: Applications include western blot analysis (1-2 µg/ml). Human cell lines including Jurkat T cells (ATCC TIB-152), 293 adenovirus-transformed kidney cells (ATCC CRL-1573) and Daudi Burkitt lymphoma cells (ATCC CCL-213) are recommended as positive controls.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Cardiovascular, Atherosclerosis, Growth Factors, Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of TRAF2. Lysates from Jurkat human T cells were probed with anti-human TRAF2 (clone C90-481) at 5.0 myg/ml (lane 1), 2.0 myg/ml (lane 2), and 1.0 myg/ml (lane 3). TRAF2 was detected at ~53 kDa

anti-TNF Receptor-Associated Factor 2 (TRAF2) (AA 93-199) antibody (Image 2)

Publications

Product: Rothe, Sarma, Dixit et al.: "TRAF2-mediated activation of NF-kappa B by TNF receptor 2 and CD40." in: Science (New York, N.Y.), Vol. 269, Issue 5229, pp. 1424-7, 1995 (PubMed).

Hsu, Shu, Pan et al.: "TRADD-TRAF2 and TRADD-FADD interactions define two distinct TNF receptor 1 signal transduction pathways." in: Cell, Vol. 84, Issue 2, pp. 299-308, 1996 (PubMed).

Shu, Takeuchi, Goeddel: "The tumor necrosis factor receptor 2 signal transducers TRAF2 and c-IAP1 are components of the tumor necrosis factor receptor 1 signaling complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 24, pp. 13973-8, 1997 (PubMed).

Tsitsikov, Wright, Geha: "CD30 induction of human immunodeficiency virus gene transcription is mediated by TRAF2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 4, pp. 1390-5, 1997 (PubMed).

Yeh, Shahinian, Speiser et al.: "Early lethality, functional NF-kappaB activation, and increased sensitivity to TNF-induced cell death in TRAF2-deficient mice." in: Immunity, Vol. 7, Issue 5, pp. 715-25, 1997 (PubMed).

Arch, Gedrich, Thompson: "Tumor necrosis factor receptor-associated factors (TRAFs)--a family of adapter proteins that regulates life and death." in: Genes & development, Vol. 12, Issue 18, pp. 2821-30, 1998 (PubMed).

Arch, Gedrich, Thompson: "Translocation of TRAF proteins regulates apoptotic threshold of cells." in: Biochemical and biophysical research communications, Vol. 272, Issue 3, pp. 936-45, 2000 (PubMed).