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Ankyrin 2, Neuronal (ANK2) antibody

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Synonyms CHANK2, ank2, zgc:101738, ANK2, AI835472, AW491075, Ank-2, ANK-2, LQT4, brank-2, Ank3, ANKB
Human, Rat (Rattus)
(86), (45), (44), (4)
(88), (3)
Clonality (Clone)
Monoclonal ()
(4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (1)
Fluorescence Microscopy (FM), ELISA, Western Blotting (WB)
(88), (80), (75), (40), (6), (4), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Quantity 0.1 mg
Shipping to United States ( )
Brand BD Pharmingen™
Immunogen Human Ankyrin B Spectrin Binding Domain Peptide
Clone 2-20
Isotype IgG1
Cross-Reactivity Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Ankyrin B (ANK2 Antibody Abstract)
Background Ankyrins are a family of proteins which were first discovered in erythrocytes and shown to bind to spectrin, a primary component of the cytoskeletal architecture. They have since been shown to be expressed at very high levels in the vertebrate brain and are also found in other cell types. Ankyrins function by acting as adaptors to link integral membrane proteins, including cell adhesion and ion channel molecules to the cytoskeleton. The structure of ankyrin consists of three domains, two highly conserved N-terminal and one C-terminal variable domain. The conserved regions contain a membrane-binding domain of ~89-95 kDa and a spectrin-binding domain of ~62 kDa, while the variable region has pre-mRNA alternative splice sites. Two ankyrins have been extensively characterized: ankryin R is the product of the ANK1 gene and is primarily expressed in the brain, although alternatively spliced forms are found in erythrocytes, ankyrin B is the product of the ANK2 gene, located on a different chromosome from ANK1, and is primarly expressed in neonatal and adult brain but is also found in heart, lymphocytes and platelets. Studies done on ankyrinB (-/-) knockout mice, which die by postnatal day 21, have demonstrated a link between the L1 cell adhesion molecule and ankyrin B in premyelinated axons. Isoforms of ankyrin B have been reported to be detectable ranging from approximately 440, 220, 150 and 110 kDa in western blot analysis, depending on the tissue-type of origin. This antibody is routinely tested by western blot analysis.
Molecular Weight 110-440 kDa
Application Notes Neonatal rat brain membranes are suggested for use as the positive control.

Related Products: ABIN967389, ABIN968545, ABIN968546

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.
Supplier Images
Western Blotting (WB) image for anti-Ankyrin 2, Neuronal (ANK2) antibody (ABIN967633) Western blot analysis of Ankyrin B. Rat brain lysates were probed with 2 µg/ml (lane ...
 image for anti-Ankyrin 2, Neuronal (ANK2) antibody (ABIN967633) anti-Ankyrin 2, Neuronal (ANK2) antibody (Image 2)
Western Blotting (WB) image for anti-Ankyrin 2, Neuronal (ANK2) antibody (ABIN967633) anti-Ankyrin 2, Neuronal (ANK2) antibody (Image 3)
Product cited in: Scotland, Zhou, Benveniste et al.: "Nervous system defects of AnkyrinB (-/-) mice suggest functional overlap between the cell adhesion molecule L1 and 440-kD AnkyrinB in premyelinated axons." in: The Journal of cell biology, Vol. 143, Issue 5, pp. 1305-15, 1999 (PubMed).

Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." in: Annual review of cell biology, Vol. 9, pp. 27-66, 1994 (PubMed).

Kordeli, Lambert, Bennett: "AnkyrinG. A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of Ranvier." in: The Journal of biological chemistry, Vol. 270, Issue 5, pp. 2352-9, 1995 (PubMed).

Kordeli, Davis, Trapp et al.: "An isoform of ankyrin is localized at nodes of Ranvier in myelinated axons of central and peripheral nerves." in: The Journal of cell biology, Vol. 110, Issue 4, pp. 1341-52, 1990 (PubMed).

Otto, Kunimoto, McLaughlin et al.: "Isolation and characterization of cDNAs encoding human brain ankyrins reveal a family of alternatively spliced genes." in: The Journal of cell biology, Vol. 114, Issue 2, pp. 241-53, 1991 (PubMed).

Catalog No. ABIN967633
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