OCT3/4A (Isoform A) antibody

Details for Product No. ABIN967679
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Isoform A
Clonality (Clone)
Monoclonal ()
Western Blotting (WB), BioImaging (BI), Intracellular Flow Cytometry (ICFC), Immunofluorescence (IF)
Pubmed 6 references available
Quantity 0.1 mg
Shipping to United States (Change)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN967679
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Human Oct3/4 Recombinant Protein
Clone O50-808
Isotype IgG1, kappa
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Background Development of a multicellular organism from a single fertilized egg is regulated by the coordinated activity of DNA transcription factors. Oct3/4, a member of the POU family of transcription factors, functions in pluripotent cells of early embryonic stem (ES) cell lines and embryonal carcinomas (EC). The human POU5F1 gene can encode various splice variants, two of which are Oct3/4A and Oct3/4B. Both isoforms share identical POU DNA-binding and C-terminal domains but differ in their N-terminal domain. The N-terminal domain of Oct3/4B is inhibitory to the DNA binding domain and therefore cannot stimulate transcription of Oct3/4-dependent genes. Oct3/4B can be detected in both pluripotent and some differentiated cell types in both the nucleus and cytoplasm, but its function is unclear. There is not an equivalent to Oct3/4B in mouse. Oct3/4A is expressed in the nucleus and has been demonstrated to orchestrate the transcription of Oct3/4-dependent genes. It has been demonstrated that the expression of Oct3/4 isoforms can vary greatly in different cell types, and discrimination of these is crucial for assessing Oct3/4 expression and function. The O50-808 monoclonal antibody recognizes human Oct3/4 Isoform A and mouse Oct3/4.
Synonyms: Oct3/4A, Oct-3A, OTF-3, NF-A3, OTF4, POU5F1, MGC22487
Molecular Weight 46 kDa
Gene ID 5460, 18999
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-OCT3/4A (Isoform A) antibody Western Blot analysis of Oct3/4 Isoform A in human embryonic stem (ES) cells. Lysate from H9 human ES cells (WiCell,Madison, WI) grown in mTESR™1 medium (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (15 µg/lane) was probed with Purified Mouse anti-Oct3/4 (Human Isoform A) monoclonal antibody at 0.125 (lane 1), 0.06 (lane 2), and 0.03 (lane 3) myg/ml. Oct3/4A is identified as a band of 46 kDa.
anti-OCT3/4A (Isoform A) antibody (2) Immunofluorescent staining of Oct3/4 Isoform A in human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) passage 33 grown on irradiated mouse fibroblasts were fixed with fixation buffer, permeabilized, and stained with Purified Mouse anti-Oct3/4 (Human Isoform A) monoclonal antibody (pseudo-colored green) at 0.06 µg/mL. The second-step reagent was AlexaFluor® 488 goat anti-mouse Ig (Life Technologies), and cell nuclei were counter stained with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. The cells were permeabilized with Phosflow Perm Buffer III, 1x Perm/Wash Buffer is also suitable for permeabilization.
Product cited in: Pan, Thomson: "Nanog and transcriptional networks in embryonic stem cell pluripotency." in: Cell research, Vol. 17, Issue 1, pp. 42-9, 2007 (PubMed).

Nishimoto, Fukushima, Okuda et al.: "The gene for the embryonic stem cell coactivator UTF1 carries a regulatory element which selectively interacts with a complex composed of Oct-3/4 and Sox-2." in: Molecular and cellular biology, Vol. 19, Issue 8, pp. 5453-65, 1999 (PubMed).

Vigano, Staudt: "Transcriptional activation by Oct-3: evidence for a specific role of the POU-specific domain in mediating functional interaction with Oct-1." in: Nucleic acids research, Vol. 24, Issue 11, pp. 2112-8, 1996 (PubMed).

Rosfjord, Scholtz, Lewis et al.: "Phosphorylation and DNA binding of the octamer binding transcription factor Oct-3." in: Biochemical and biophysical research communications, Vol. 212, Issue 3, pp. 847-53, 1995 (PubMed).

Yuan, Corbi, Basilico et al.: "Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3." in: Genes & development, Vol. 9, Issue 21, pp. 2635-45, 1995 (PubMed).

Okamoto, Okazawa, Okuda et al.: "A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells." in: Cell, Vol. 60, Issue 3, pp. 461-72, 1990 (PubMed).

Validation Images
Did you look for something else?
back to top