Forkhead Box A2 (FOXA2) antibody

Details for Product No. ABIN967680
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Antigen
Synonyms
HNF3B, TCF3B, Hnf3b, Tcf3b, Hnf-3b, Tcf-3b, HNF3beta, HNF3-beta, HNF-3B, ax, axl, fkd1, ik:tdsubc_2g8, tdsubc_2g8, wu:fe05g04, xx:tdsubc_2g8, HNF-3BETA, HNF-3b, hnf3b, xFD-3, FoxA2a, FoxA2-A, xbeta-1, ... show more
HNF3B, TCF3B, Hnf3b, Tcf3b, Hnf-3b, Tcf-3b, HNF3beta, HNF3-beta, HNF-3B, ax, axl, fkd1, ik:tdsubc_2g8, tdsubc_2g8, wu:fe05g04, xx:tdsubc_2g8, HNF-3BETA, HNF-3b, hnf3b, xFD-3, FoxA2a, FoxA2-A, xbeta-1, hnf3beta, hnf3-beta, tcf3b, FOXA2 show less
Reactivity
Human
(84), (24), (19), (13), (12), (12), (3), (1), (1)
Host
Mouse
(42), (39), (10)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), BioImaging (BI), Intracellular Flow Cytometry (ICFC)
(81), (47), (27), (23), (18), (10), (8), (5), (2), (2), (1), (1)
Pubmed 7 references available
Catalog no. ABIN967680
Quantity 0.1 mg
Price
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Immunogen Human FoxA2 Peptide
Clone N17-280
Isotype IgG1, kappa
Cross-Reactivity (Details) Predicted: Mouse
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Triton is a trademark of the Dow Chemical Company.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name FOXA2
Background FoxA2, forkhead box A2, is a member of the forkhead class of DNA-binding proteins that regulates gene expression in the liver, pancreatic islets, adipocytes and some neural cells. This hepatocyte nuclear factor is a transcriptional activator for liver-specific genes such as alpha fetoprotein, albumin, tyrosine aminotransferase and transthyretin. FoxA2 is expressed in embryonic endoderm, the germ layer that gives rise to the digestive system, and contributes to the specification of the pancreas and the regulation of glucose homoeostasis. FoxA2 also has roles in neural development. Specifically, FoxA2 cooperates with related FoxA1 in the specification and differentiation of midbrain dopaminergic neurons in a dosage-dependent manner.
Synonyms: Forkhead box A2, HNF3B, HNF-3B, HNF-3beta, hepatocyte nuclear factor 3beta
Molecular Weight 48 kDa
Research Area Lineage Markers, Transcription Factors
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Forkhead Box A2 (FOXA2) antibody Western blot analysis of FoxA2 in definitive endoderm derived from human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) were differentiated to definitive endoderm for 3 days (D’Amour et al, 2005) in RPMI medium supplemented with 0.5% FBS, 1X L-glutamine, and 100 ng/ml Activin A (R&D Systems). Lysates from control ES cells (lane 1) and from day 1 (lane 2) and day 3 (lane 3) differentiated cells were probed with Purified Mouse anti-Human FoxA2 antibody at 1.0 µg/ml. The presence of FoxA2 is demonstrated by the 48-kDa band in human ES-derived definitive endodermal cells (Lane 3), which is absent in H9 human ES cells (Lane 1) and at day 1 of differentiation (Lane 2). Purified Mouse anti-Hsp90 monoclonal antibody (ABIN967957) was used as a gel-loading control (MW 90 kDa).
anti-Forkhead Box A2 (FOXA2) antibody (2) Immunofluorescent staining of FoxA2 in definitive endoderm derived from human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) passage 35 grown on an irradiated mouse embryonic feeder layer were differentiated to definitive endoderm for 3 days (D’Amour et al, 2005) in RPMI medium supplemented with 0.5% FBS,1x L-glutamine, and 100 ng/ml Activin A (R&D Systems). The cells were fixed with BD Cytofix buffer, permeabilized with 0.1% Triton™ X-100, and stained with Purified Mouse anti-Human FoxA2 monoclonal antibody (pseudo-colored green) at 5 µg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter staining was with Hoechst 33342 (pseudo-colored blue). The image was captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software.
Product cited in: Lai, Prezioso, Tao et al.: "Hepatocyte nuclear factor 3 alpha belongs to a gene family in mammals that is homologous to the Drosophila homeotic gene fork head." in: Genes & development, Vol. 5, Issue 3, pp. 416-27, 1991 (PubMed).

Clevidence, Overdier, Tao et al.: "Identification of nine tissue-specific transcription factors of the hepatocyte nuclear factor 3/forkhead DNA-binding-domain family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 9, pp. 3948-52, 1993 (PubMed).

Monaghan, Kaestner, Grau et al.: "Postimplantation expression patterns indicate a role for the mouse forkhead/HNF-3 alpha, beta and gamma genes in determination of the definitive endoderm, chordamesoderm and neuroectoderm." in: Development (Cambridge, England), Vol. 119, Issue 3, pp. 567-78, 1994 (PubMed).

Thomson, Itskovitz-Eldor, Shapiro et al.: "Embryonic stem cell lines derived from human blastocysts." in: Science (New York, N.Y.), Vol. 282, Issue 5391, pp. 1145-7, 1998 (PubMed).

DAmour, Agulnick, Eliazer et al.: "Efficient differentiation of human embryonic stem cells to definitive endoderm." in: Nature biotechnology, Vol. 23, Issue 12, pp. 1534-41, 2005 (PubMed).

Burtscher, Lickert: "Foxa2 regulates polarity and epithelialization in the endoderm germ layer of the mouse embryo." in: Development (Cambridge, England), Vol. 136, Issue 6, pp. 1029-38, 2009 (PubMed).

Lin, Metzakopian, Mavromatakis et al.: "Foxa1 and Foxa2 function both upstream of and cooperatively with Lmx1a and Lmx1b in a feedforward loop promoting mesodiencephalic dopaminergic neuron development." in: Developmental biology, Vol. 333, Issue 2, pp. 386-96, 2009 (PubMed).

Hosts (42), (39), (10)
Reactivities (84), (24), (19), (13), (12), (12), (3), (1), (1)
Applications (81), (47), (27), (23), (18), (10), (8), (5), (2), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (13), (12), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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