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ROR GAM T antibody

Details for Product No. ABIN967684, Supplier: Log in to see
Antigen
Reactivity
Human, Mouse (Murine)
Host
Mouse
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
Supplier
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Brand BD Pharmingen™
Immunogen Human RORgammat Recombinant Protein
Clone O28-835
Isotype IgG2b, kappa
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Background The O28-835 monoclonal antibody recognizes ROR-gamma (RORgamma) and its isoform, RORgammat. RORgamma (~58 kDa calculated molecular weight) is a DNA-binding transcription factor that belongs to the ROR/RZR orphan nuclear receptor family. It is expressed in different tissues including skeletal muscle, kidney, liver and adipose tissues. RORgammat (~56 kDa calculated molecular weight) is a RORgamma isoform that is expressed exclusively by lymphoid cells. It is expressed by various lymphoid cell types including CD4+CD8+ thymocytes, peripheral CD4+ Th17 and CD8+ Tc17 cells and lymphoid tissue inducer (Lti) cells. RORgammat plays essential roles in thymopoiesis, T cell homeostasis, differentiation of effector T lymphocytes and the development of secondary lymphoid tissues including lymph nodes and Peyer’s patches.
Synonyms: Rorc, Rorg, RORgamma, Rorgamma, RORgt, RORgammat, RORgammat, TOR, Thor, Nr1f3, ROR
Molecular Weight 56-58 kDa
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-ROR GAM T antibody (ABIN967684) Western blot analysis of RORgammat expression by mouse thymocytes. Cell lysate was pr...
Product cited in: Hamada, Garcia-Hernandez, Reome, Misra, Strutt, McKinstry, Cooper, Swain, Dutton: "Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 6, pp. 3469-81, 2009 (PubMed).

Ivanov, McKenzie, Zhou, Tadokoro, Lepelley, Lafaille, Cua, Littman: "The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells." in: Cell, Vol. 126, Issue 6, pp. 1121-33, 2006 (PubMed).

Eberl, Littman: "The role of the nuclear hormone receptor RORgammat in the development of lymph nodes and Peyer's patches." in: Immunological reviews, Vol. 195, Issue 9, pp. 81-90, 2003 (PubMed).

Eberl, Marmon, Sunshine, Rennert, Choi, Littman: "An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells." in: Nature immunology, Vol. 5, Issue 1, pp. 64-73, 2003 (PubMed).

He, Deftos, Ojala, Bevan: "RORgamma t, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells." in: Immunity, Vol. 9, Issue 6, pp. 797-806, 1999 (PubMed).

Medvedev, Yan, Hirose, Giguère, Jetten: "Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element." in: Gene, Vol. 181, Issue 1-2, pp. 199-206, 1997 (PubMed).