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Epidermal Growth Factor Receptor Pathway Substrate 8 (EPS8) (AA 628-821) antibody

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Synonyms MGC81285, MGC146320, AW261790
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AA 628-821
(22), (16), (13), (3), (2), (2), (2), (1), (1), (1), (1)
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(69), (26), (23), (4), (3), (2), (2), (2), (1)
(52), (15), (4), (1)
Clonality (Clone)
Monoclonal ()
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(43), (36), (31), (12), (11), (6), (3), (3), (1), (1), (1)
Pubmed 4 references available
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Quantity 50 μg
Shipping to United States ( )
Immunogen Mouse Eps8 aa. 628-821
Clone 15-Eps8
Isotype IgG1
Cross-Reactivity Human, Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Eps8 (EPS8 Antibody Abstract)
Background The p97 [Eps8] protein, a substrate for EGF-R tyrosine kinase, contains an SH3 domain, but lacks a functional SH2 domain. Antibodies to Eps8 recognize the 97 kDa protein and a less abundant 68 kDa protein. Both forms are tyrosine-phosphorylated following treatment of cells with EGF. It is likely that p68 [Eps8] is synthesized from an alternatively spliced mRNA since two major Eps8-specific mRNAs are detected by Northern analysis. Co-immunoprecipitation studies have demonstrated a physical association between the Eps8 protein and the EGF-R both in vivo and in vitro. For many EGF-R substrates, this interaction is mediated through an SH2 domain of the substrate. Since Eps8 lacks a well defined SH2 domain and a fusion protein containing the SH2-like region of Eps8 could not bind EGF-R, the mechanism of Eps8-EGF-R association remains unclear. Overexpression of Eps8 in fibroblasts and hematopoietic cells results in an increased mitogenic response to EGF, suggesting that Eps8 has a role in the modulation of EGF-R function.
Molecular Weight 97 kDa
Pathways EGFR Signaling Pathway

Related Products: ABIN967389, ABIN968550

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor Pathway Substrate 8 (EPS8) (AA 628-821) antibody (ABIN967774) Western blot analysis of Eps8 on a lysate from mouse macrophages (RAW 264.7) treated ...
Immunofluorescence (IF) image for anti-Epidermal Growth Factor Receptor Pathway Substrate 8 (EPS8) (AA 628-821) antibody (ABIN967774) Immunofluorescence staining of rabbit cerebellum.
Product cited in: Burke, Schooler, Wiley: "Regulation of epidermal growth factor receptor signaling by endocytosis and intracellular trafficking." in: Molecular biology of the cell, Vol. 12, Issue 6, pp. 1897-910, 2001 (PubMed).

Miyamoto, Teramoto, Gutkind et al.: "Integrins can collaborate with growth factors for phosphorylation of receptor tyrosine kinases and MAP kinase activation: roles of integrin aggregation and occupancy of receptors." in: The Journal of cell biology, Vol. 135, Issue 6 Pt 1, pp. 1633-42, 1997 (PubMed).

Xie, Cho, Calaycay et al.: "Cloning and characterization of inducible nitric oxide synthase from mouse macrophages." in: Science (New York, N.Y.), Vol. 256, Issue 5054, pp. 225-8, 1992 (PubMed).

Background publications Fazioli, Minichiello, Matoska et al.: "Eps8, a substrate for the epidermal growth factor receptor kinase, enhances EGF-dependent mitogenic signals." in: The EMBO journal, Vol. 12, Issue 10, pp. 3799-808, 1993 (PubMed).

Catalog No. ABIN967774
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