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Phosphotyrosine antibody

Details for Product No. ABIN967962, Supplier: Login to see
Antigen
Reactivity
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
124
51
43
29
28
27
24
8
8
5
1
Host
Mouse
193
63
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
28
26
18
15
9
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
190
113
93
78
76
56
28
27
11
8
7
2
2
1
1
1
1
Supplier
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Clone PY69
Isotype IgG2a
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Background Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins.
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Pathways
Application Notes The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Aqueous buffered solution containing glycerol and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Phosphotyrosine antibody (ABIN967962) Western blot analysis of Phosphotyrosine on A431 lysate. 1:1000 (lane 1), 1:2000 (lan...
 image for anti-Phosphotyrosine antibody (ABIN967962) Phosphotyrosine, clone PY69. First Panel: A431 control. Second Panel: A431 + EGF
Product cited in: Doronin, Shumay, Wang et al.: "Akt mediates sequestration of the beta(2)-adrenergic receptor in response to insulin." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 15124-31, 2002 (PubMed).

Doronin, Wang Hy, Malbon: "Insulin stimulates phosphorylation of the beta 2-adrenergic receptor by the insulin receptor, creating a potent feedback inhibitor of its tyrosine kinase." in: The Journal of biological chemistry, Vol. 277, Issue 12, pp. 10698-703, 2002 (PubMed).

Moro, Dolce, Cabodi et al.: "Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosines." in: The Journal of biological chemistry, Vol. 277, Issue 11, pp. 9405-14, 2002 (PubMed).

Yan, Deng, Friedman: "Oncogenic Ki-ras confers a more aggressive colon cancer phenotype through modification of transforming growth factor-beta receptor III." in: The Journal of biological chemistry, Vol. 276, Issue 2, pp. 1555-63, 2001 (PubMed).

Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, 1988 (PubMed).