Phosphotyrosine antibody

Antigen: Phosphotyrosine

Clonality: Monoclonal (PY69)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Chicken, Dog (Canine), Frog

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN967962

Additional Information

Format: Liquid

Isotype: IgG2a

Clone: PY69

Description: Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Application Details

Application Notes: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes.

Concentration: 1.0 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling, Metabolism, Amino Acids, Protein Modifications

Restrictions: For Research Use only

Images

Western blot analysis of Phosphotyrosine on A431 lysate. 1:1000 (lane 1), 1:2000 (lane 2), 1:4000 (lane 3) dilution of Phosphotyrosine.

Phosphotyrosine, clone PY69. First Panel: A431 control. Second Panel: A431 + EGF

Publications

Product: Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, 1988 (PubMed).

Yan, Deng, Friedman: "Oncogenic Ki-ras confers a more aggressive colon cancer phenotype through modification of transforming growth factor-beta receptor III." in: The Journal of biological chemistry, Vol. 276, Issue 2, pp. 1555-63, 2001 (PubMed).

Moro, Dolce, Cabodi et al.: "Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosines." in: The Journal of biological chemistry, Vol. 277, Issue 11, pp. 9405-14, 2002 (PubMed).

Doronin, Wang Hy, Malbon: "Insulin stimulates phosphorylation of the beta 2-adrenergic receptor by the insulin receptor, creating a potent feedback inhibitor of its tyrosine kinase." in: The Journal of biological chemistry, Vol. 277, Issue 12, pp. 10698-703, 2002 (PubMed).

Doronin, Shumay, Wang et al.: "Akt mediates sequestration of the beta(2)-adrenergic receptor in response to insulin." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 15124-31, 2002 (PubMed).