Phosphotyrosine antibody

Details for Product No. ABIN967962
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Reactivity
Human, Mouse (Murine), Rat (Rattus), Chicken, Dog (Canine), Frog
(163), (38), (24), (20), (16), (10), (4), (1), (1), (1)
Host
Mouse
(177), (45), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(28), (21), (18), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
(165), (112), (89), (33), (32), (24), (20), (16), (15), (10), (3), (2), (1), (1), (1)
Pubmed 5 references available
Catalog no. ABIN967962
Quantity 1 mg
Price
Contact our Customer Service for availability and price in your country.
Options
Shipping to United States (Change)
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Clone PY69
Isotype IgG2a
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Background Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins.
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Application Notes The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/ml
Buffer Aqueous buffered solution containing glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Phosphotyrosine antibody Western blot analysis of Phosphotyrosine on A431 lysate. 1:1000 (lane 1), 1:2000 (lane 2), 1:4000 (lane 3) dilution of Phosphotyrosine.
anti-Phosphotyrosine antibody (2) Phosphotyrosine, clone PY69. First Panel: A431 control. Second Panel: A431 + EGF
Product cited in: Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, 1988 (PubMed).

Yan, Deng, Friedman: "Oncogenic Ki-ras confers a more aggressive colon cancer phenotype through modification of transforming growth factor-beta receptor III." in: The Journal of biological chemistry, Vol. 276, Issue 2, pp. 1555-63, 2001 (PubMed).

Moro, Dolce, Cabodi et al.: "Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosines." in: The Journal of biological chemistry, Vol. 277, Issue 11, pp. 9405-14, 2002 (PubMed).

Doronin, Wang Hy, Malbon: "Insulin stimulates phosphorylation of the beta 2-adrenergic receptor by the insulin receptor, creating a potent feedback inhibitor of its tyrosine kinase." in: The Journal of biological chemistry, Vol. 277, Issue 12, pp. 10698-703, 2002 (PubMed).

Doronin, Shumay, Wang et al.: "Akt mediates sequestration of the beta(2)-adrenergic receptor in response to insulin." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 15124-31, 2002 (PubMed).

Hosts (177), (45), (2)
Reactivities (163), (38), (24), (20), (16), (10), (4), (1), (1), (1)
Applications (165), (112), (89), (33), (32), (24), (20), (16), (15), (10), (3), (2), (1), (1), (1)
Conjugates (28), (21), (18), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (8), (5), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top