Syntaxin 4 (STX4) (AA 1-280) antibody

Antigen: Syntaxin 4 (STX4)

Synonyms: STX4A, p35-2, Dm Syx4, DmSyx4, EG:95B7.1, EG:95B7.11, Syx, Syx-4, syx4, DmelCG2715, CG2715, Stx4a, STX4, stx4a, MGC89357, MGC128969, MGC130995

Binding Site: AA 1-280

Clonality: Monoclonal (49)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Catalog no.: ABIN967968

Additional Information

Alternative name: Syntaxin 4

Immunogen: Human Syntaxin 4

Format: Liquid

Isotype: IgG1

Clone: 49

Description: Signal transmission between neurons is regulated by the release of neurotransmitters at the synapse. This process is controlled by a complex pathway of membrane trafficking in the presynaptic nerve terminal, which leads to membrane fusion and subsequent neurotransmitter secretion. Syntaxin is involved in three important protein complexes that modulate this process: syntaxin and n-sec1, syntaxin, VAMP and SNAP-25, and syntaxin, VAMP, SNAP-25, alphaSNAP, and NSF (20S complex). A model has been proposed to explain docking, activation, and fusion of synaptic vesicles with donor membranes. This model suggests that VAMP/synaptobrevin and synaptotagmin (vSNARE) on the synaptic vesicle, and SNAP-25 and syntaxin (tSNAREs) on the plasma membrane, interact to form a 7S complex. It appears that syntaxin associates with -sec1 prior to and/or during the formation of the 7S complex. Two additional soluble proteins, alphaSNAP and NSF, associate with this complex as synaptotagmin releases from the complex. The resulting 20S complex contains syntaxin, SNAP-25, VAMP, alphaSNAP, and NSF. A large region including the N-terminus is involved in binding n-sec1 while both VAMP and SNAP-25 bind within residues 199-288 of syntaxin.
Synonyms: STX4

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to us.

Molecular Weight: 32 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling

Restrictions: For Research Use only

Images

Western blot analysis of Syntaxin 4 on human endothelial lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of anti-syntaxin 4.

Immunofluorescent staining on Human Intestinal Smooth Muscle (HISM) cells.

Publications

Product: Bennett, Calakos, Scheller: "Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones." in: Science (New York, N.Y.), Vol. 257, Issue 5067, pp. 255-9, 1992 (PubMed).

Kee, Lin, Hsu et al.: "Distinct domains of syntaxin are required for synaptic vesicle fusion complex formation and dissociation." in: Neuron, Vol. 14, Issue 5, pp. 991-8, 1995 (PubMed).

Ramalho-Santos, Moreno, Sutovsky et al.: "SNAREs in mammalian sperm: possible implications for fertilization." in: Developmental biology, Vol. 223, Issue 1, pp. 54-69, 2000 (PubMed).

Faigle, Colucci-Guyon, Louvard et al.: "Vimentin filaments in fibroblasts are a reservoir for SNAP23, a component of the membrane fusion machinery." in: Molecular biology of the cell, Vol. 11, Issue 10, pp. 3485-94, 2000 (PubMed).

Polgár, Chung, Reed: "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion." in: Blood, Vol. 100, Issue 3, pp. 1081-3, 2002 (PubMed).