Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2) (AA 254-497) antibody

Antigen: Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2)

Synonyms: RCH2, SRP1, IPOA5, NPI-1, QIP2, RCH1, IPOA1, SRP1alpha, Gm1055, AW146299, KPNA1, DKFZp459M124, ima2

Binding Site: AA 254-497

Clonality: Monoclonal (2)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN967998

Additional Information

Alternative name: Karyopherin alpha 2

Immunogen: Human Rch-1

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Fruit Fly (Drosophila melanogaster)

Format: Liquid

Isotype: IgG1

Clone: 2

Description: The two step process of importing proteins into the nucleus involves the binding and interaction of several cytosolic and nuclear pore proteins. Proteins to be translocated into the nucleus contain a nuclear localization sequence (NLS) which is recognized and bound by carrier proteins in the cytosol. Heterodimers belonging to a highly conserved family of proteins called karyopherins are required for successful nuclear localization of cytosolic proteins. The alpha-subunits appear to function in the binding of NLS (both simple and bitartite NLS motifs), but both alpha- and beta-subunits are required for successful docking to the nuclear envelope. ATP is required for complete translocation of proteins into the nucleus. Karyopherin alpha2 was first identified as Rch-1, an NLS receptor which interacts with the RAG-1 recombination-activating protein in developing B and T cells. Rch-1 has been reported to be 44% identical to karyopherin alpha1 (hSRP-1 /NPI-1). This antibody is routinely tested by western blot analysis.
Synonyms: Rch-1

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 58 kDa

Comments:

Related Products: ABIN968535, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of Karyopherin alpha on a HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2.2). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti-karyopherin antibody.

Immunofluorescence staining of WI-38 cells (Human lung fibroblasts, ATCC CCL-75).

Publications

Product: Moroianu, Hijikata, Blobel et al.: "Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal and beta subunit interacts with peptide repeat-containing nucleoporins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 14, pp. 6532-6, 1995 (PubMed).

Weis, Mattaj, Lamond: "Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences." in: Science (New York, N.Y.), Vol. 268, Issue 5213, pp. 1049-53, 1995 (PubMed).

Cuomo, Kirch, Gyuris et al.: "Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 13, pp. 6156-60, 1994 (PubMed).

Grozinger, Schreiber: "Regulation of histone deacetylase 4 and 5 and transcriptional activity by 14-3-3-dependent cellular localization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 14, pp. 7835-40, 2000 (PubMed).