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PKA R1 alpha (PKR1) (AA 1-381) antibody

Reactivity: Chicken, Dog, Human, Mouse, Rat IF, IP, IHC, WB Host: Mouse Monoclonal 20-PKA RIalpha unconjugated
Catalog No. ABIN968062
  • Target
    PKA R1 alpha (PKR1)
    Binding Specificity
    AA 1-381
    Reactivity
    Chicken, Dog, Human, Mouse, Rat
    Host
    Mouse
    Clonality
    Monoclonal
    Application
    Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
    Cross-Reactivity
    Rat (Rattus), Dog (Canine), Mouse (Murine), Chicken
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human PKA [RIalpha] aa. 1-381
    Clone
    20-PKA RIalpha
    Isotype
    IgG1
  • Comment

    Related Products: ABIN968545, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20°C.
  • Taskén, Collas, Kemmner, Witczak, Conti, Taskén: "Phosphodiesterase 4D and protein kinase a type II constitute a signaling unit in the centrosomal area." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 21999-2002, (2001) (PubMed).

    Vang, Torgersen, Sundvold, Saxena, Levy, Skålhegg, Hansson, Mustelin, Taskén: "Activation of the COOH-terminal Src kinase (Csk) by cAMP-dependent protein kinase inhibits signaling through the T cell receptor." in: The Journal of experimental medicine, Vol. 193, Issue 4, pp. 497-507, (2001) (PubMed).

    Casey, Vaughan, He, Hatcher, Winter, Weremowicz, Montgomery, Kucherlapati, Morton, Basson: "Mutations in the protein kinase A R1alpha regulatory subunit cause familial cardiac myxomas and Carney complex." in: The Journal of clinical investigation, Vol. 106, Issue 5, pp. R31-8, (2000) (PubMed).

    Skålhegg, Landmark, Foss, Lohmann, Hansson, Lea, Jahnsen: "Identification, purification, and characterization of subunits of cAMP-dependent protein kinase in human testis. Reverse mobilities of human RII alpha and RII beta on sodium dodecyl sulfate-polyacrylamide gel electrophoresis compared with rat and bovine R" in: The Journal of biological chemistry, Vol. 267, Issue 8, pp. 5374-9, (1992) (PubMed).

    Sandberg, Skålhegg, Jahnsen: "The two mRNA forms for the type I alpha regulatory subunit of cAMP-dependent protein kinase from human testis are due to the use of different polyadenylation site signals." in: Biochemical and biophysical research communications, Vol. 167, Issue 1, pp. 323-30, (1990) (PubMed).

  • Target
    PKA R1 alpha (PKR1)
    Alternative Name
    PKA RI alpha
    Background
    CAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIalpha, RIbeta, RIIalpha, and RIIbeta. These subunits define type I and II cAMP-dependent protein kinases. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Calpha, Cbeta, or Cgamma). Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity. Most cells express both type I and type II PKAs. Although the Ralpha isoforms are ubiquitously expressed, the Rbeta isoforms are predominant in nervous and adipose tissues. Expression of the RIbeta subunit is modulated during muscle and adipocyte differentiation in vitro.
    Molecular Weight
    49 kDa
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