A Kinase (PRKA) Anchor Protein 1 (AKAP1) (AA 66-212) antibody

Details for Product No. ABIN968136
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Antigen
Synonyms AKAP, AKAP121, AKAP149, AKAP84, D-AKAP1, PPP1R43, PRKA1, SAKAP84, TDRD17, Akap, C76494, C81186, DAKAP1, S-AKAP84, Akap84, AKAP1, akap1, fa08b04, wu:fa08b04
Epitope
AA 66-212
(2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(20)
Host
Mouse
(18), (3)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
(21), (14), (11), (10), (4), (2), (1)
Pubmed 5 references available
Catalog no. ABIN968136
Quantity 50 µg
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Immunogen Human AKAP149
Clone 6
Isotype IgG1
Cross-Reactivity Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name AKAP149
Background The targeting of protein kinases and phosphatases to cellular compartments in a timely manner may provide specificity in signal transduction events. For instance, attachment of type II cAMP-dependent Protein Kinase (PKA) to the cytoskeleton occurs through the binding of the regulatory subunit (RII) to microtubule associated-protein 2. Several proteins, such as microtubule-associated protein 2, have been identified as PKA type I/II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). AKAP149 is a protein of 903 amino acids. It has a PKA [RII] binding site at amino acids 355-376 preceded by a leucine zipper domain. AKAP149 mRNA has been reported to be expressed ubiquitously, with the highest levels in prostate and small intestine. AKAP149 contains a KH domain characteristic of RNA-binding proteins. Thus, AKAP149 is a PKA [RII] anchoring protein that may target this kinase to sites of RNA processing for regulation of RNA binding protein activity. This antibody is routinely tested by western blot analysis.
Molecular Weight 149 kDa
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Supplier Images
anti-A Kinase (PRKA) Anchor Protein 1 (AKAP1) (AA 66-212) antibody Western blot analysis of AKAP149 on a HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2.2). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- AKAP149 antibody.
anti-A Kinase (PRKA) Anchor Protein 1 (AKAP1) (AA 66-212) antibody (2) Immunofluorescence staining of A431 cells (Human epithelial carcinoma, ATCC CRL-1555).
anti-A Kinase (PRKA) Anchor Protein 1 (AKAP1) (AA 66-212) antibody (3) anti-A Kinase (PRKA) Anchor Protein 1 (AKAP1) (AA 66-212) antibody (Image 3)
Product cited in: Trendelenburg, Hummel, Riecken et al.: "Molecular characterization of AKAP149, a novel A kinase anchor protein with a KH domain." in: Biochemical and biophysical research communications, Vol. 225, Issue 1, pp. 313-9, 1996 (PubMed).

DellAcqua, Scott: "Protein kinase A anchoring." in: The Journal of biological chemistry, Vol. 272, Issue 20, pp. 12881-4, 1997 (PubMed).

Steen, Collas: "Mistargeting of B-type lamins at the end of mitosis: implications on cell survival and regulation of lamins A/C expression." in: The Journal of cell biology, Vol. 153, Issue 3, pp. 621-6, 2001 (PubMed).

Schillace, Andrews, Liberty et al.: "Identification and characterization of myeloid translocation gene 16b as a novel a kinase anchoring protein in T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 4, pp. 1590-9, 2002 (PubMed).

Tanji, Yamamoto, Yorioka et al.: "A-kinase anchoring protein AKAP220 binds to glycogen synthase kinase-3beta (GSK-3beta ) and mediates protein kinase A-dependent inhibition of GSK-3beta." in: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 36955-61, 2002 (PubMed).

Hosts (18), (3)
Reactivities (20)
Applications (21), (14), (11), (10), (4), (2), (1)
Epitopes (2), (2), (2), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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