anti-AKAP1 antibody (A Kinase (PRKA) Anchor Protein 1) (AA 66-212)

Details for Product anti-AKAP1 Antibody No. ABIN968136
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Synonyms AKAP1, akap1, fa08b04, wu:fa08b04, AKAP, AKAP121, AKAP149, AKAP84, D-AKAP1, PPP1R43, PRKA1, SAKAP84, TDRD17, Akap, C76494, C81186, DAKAP1, S-AKAP84, Akap84
AA 66-212
(3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Rat (Rattus)
(38), (3), (3)
(33), (5)
Clonality (Clone)
Monoclonal ()
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(34), (23), (19), (17), (6), (3), (3), (1)
Pubmed 5 references available
Quantity 50 μg
Shipping to United States ( )
Immunogen Human AKAP149 aa. 66-212
Clone 6-AKAP149
Isotype IgG1
Cross-Reactivity Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name AKAP149 (AKAP1 Antibody Abstract)
Background The targeting of protein kinases and phosphatases to cellular compartments in a timely manner may provide specificity in signal transduction events. For instance, attachment of type II cAMP-dependent Protein Kinase (PKA) to the cytoskeleton occurs through the binding of the regulatory subunit (RII) to microtubule associated-protein 2. Several proteins, such as microtubule-associated protein 2, have been identified as PKA type I/II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). AKAP149 is a protein of 903 amino acids. It has a PKA [RII] binding site at amino acids 355-376 preceded by a leucine zipper domain. AKAP149 mRNA has been reported to be expressed ubiquitously, with the highest levels in prostate and small intestine. AKAP149 contains a KH domain characteristic of RNA-binding proteins. Thus, AKAP149 is a PKA [RII] anchoring protein that may target this kinase to sites of RNA processing for regulation of RNA binding protein activity. This antibody is routinely tested by western blot analysis.
Molecular Weight 149 kDa

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-AKAP1 antibody (A Kinase (PRKA) Anchor Protein 1) (AA 66-212) (ABIN968136) Western blot analysis of AKAP149 on a HeLa cell lysate (Human cervical epitheloid car...
Immunofluorescence (IF) image for anti-AKAP1 antibody (A Kinase (PRKA) Anchor Protein 1) (AA 66-212) (ABIN968136) Immunofluorescence staining of A431 cells (Human epithelial carcinoma, ATCC CRL-1555).
Image no. 3 for anti-AKAP1 antibody (A Kinase (PRKA) Anchor Protein 1) (AA 66-212) (ABIN968136) anti-A Kinase (PRKA) Anchor Protein 1 (AKAP1) (AA 66-212) antibody (Image 3)
Product cited in: Tanji, Yamamoto, Yorioka et al.: "A-kinase anchoring protein AKAP220 binds to glycogen synthase kinase-3beta (GSK-3beta ) and mediates protein kinase A-dependent inhibition of GSK-3beta." in: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 36955-61, 2002 (PubMed).

Schillace, Andrews, Liberty et al.: "Identification and characterization of myeloid translocation gene 16b as a novel a kinase anchoring protein in T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 4, pp. 1590-9, 2002 (PubMed).

Steen, Collas: "Mistargeting of B-type lamins at the end of mitosis: implications on cell survival and regulation of lamins A/C expression." in: The Journal of cell biology, Vol. 153, Issue 3, pp. 621-6, 2001 (PubMed).

DellAcqua, Scott: "Protein kinase A anchoring." in: The Journal of biological chemistry, Vol. 272, Issue 20, pp. 12881-4, 1997 (PubMed).

Trendelenburg, Hummel, Riecken et al.: "Molecular characterization of AKAP149, a novel A kinase anchor protein with a KH domain." in: Biochemical and biophysical research communications, Vol. 225, Issue 1, pp. 313-9, 1996 (PubMed).

Catalog No. ABIN968136
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