Ron alpha (AA 40-224) antibody

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AA 40-224
Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
Clonality (Clone)
Monoclonal ()
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen Human RONalpha aa. 40-224
Clone 29-RONalpha
Isotype IgG2b
Cross-Reactivity Dog (Canine), Rat (Rattus), Mouse (Murine), Frog
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Background RON (Recepteur d'Origine Nantaise) is a tyrosine kinase receptor for MSP (Macrophage Stimulating Protein) that belongs to the hepatocyte growth factor receptor family. RON is synthesized as a single chain glycosylated polypeptide of 1400 amino acids that is cleaved upon maturation into a 150 kDa beta-subunit with the kinase activity and a 40 kDa alpha-chain. Both chains are linked by disulfide bonds and the proper arrangement of the alpha and beta heterodimer is necessary for biological activity of RON. MSP induces the tyrosine kinase activity of RON, triggering a cascade of intracellular signaling that leads to DNA synthesis. However, RON activation in mouse erythroleukemia cells results in apoptosis. The replacement of autophosphorylation sites Y1330 and Y1337 abolishes both the mitogenic and apoptotic effects of MSP. Therefore, autophosphorylation at Y1330 and Y1337 is required for RON's biological activity. PLCgamma, Shc, Grb2, p61, and p65 associate with RON. Although widely expressed, RON is particularly abundant in epithelial tissues, lung, brain, adrenal glands, gastrointestinal tract, kidney, and testis.
Synonyms: Recepteur d'Origine Nantaise
Molecular Weight 40 kDa

Related Products: ABIN968619, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Ron alpha (AA 40-224) antibody (ABIN968150) Western blot analysis of RONalpha on a MDCK cell lysate (Canine kidney, ATCC CCL-34)....
Immunofluorescence (IF) image for anti-Ron alpha (AA 40-224) antibody (ABIN968150) Immunofluorescence staining of HS 766T cells (Human pancreatic carcinoma, ATCC HTB-134).
Product cited in: Peace, Hughes, Degen, Waltz: "Point mutations and overexpression of Ron induce transformation, tumor formation, and metastasis." in: Oncogene, Vol. 20, Issue 43, pp. 6142-51, 2001 (PubMed).

Gaudino, Follenzi, Naldini, Collesi, Santoro, Gallo, Godowski, Comoglio: "RON is a heterodimeric tyrosine kinase receptor activated by the HGF homologue MSP." in: The EMBO journal, Vol. 13, Issue 15, pp. 3524-32, 1994 (PubMed).

Wang, Ronsin, Gesnel, Coupey, Skeel, Leonard, Breathnach: "Identification of the ron gene product as the receptor for the human macrophage stimulating protein." in: Science (New York, N.Y.), Vol. 266, Issue 5182, pp. 117-9, 1994 (PubMed).

Ronsin, Muscatelli, Mattei, Breathnach: "A novel putative receptor protein tyrosine kinase of the met family." in: Oncogene, Vol. 8, Issue 5, pp. 1195-202, 1993 (PubMed).