X-Linked Inhibitor of Apoptosis (XIAP) (AA 268-426) antibody

Antigen: X-Linked Inhibitor of Apoptosis (XIAP)

Synonyms: API3, ILP1, MIHA, XLP2, BIRC4, XIAP, birc4, xiap, MGC107868, xxiap

Binding Site: AA 268-426

Clonality: Monoclonal (48)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN968163

Additional Information

Alternative name: hILP

Immunogen: Human hILP

Cross-Reactivity: Rat (Rattus), Mouse (Murine), Frog

Format: Liquid

Isotype: IgG1

Clone: 48

Description: Apoptosis is a genetically programmed, selective process of cell death that occurs during normal cell differentiation and development of multicellular organisms. Viruses depend on the biosynthetic machinery of their host cell for the production of progeny and survival. Therefore, many viruses encode proteins that protect the cell from apoptosis. hILP (human IAP-like protein) is a human homologue of the viral IAP (Inhibitor of Apoptosis Protein). hILP is a widely expressed cytoplasmic protein of 497 amino acids with three BIR (Baculovirus IAP repeats) domains and a C-terminal RING finger domain. hILP-transfected cells are protected against the apoptotic effects of Sindbis virus infection and ICE (interleukin-1beta converting enzyme) expression. This product is sold under license from Aegera Therapeutics, Inc. This antibody is routinely tested by western blot analysis.
Synonyms: hILP

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 57 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of hILP/XIAP on Jurkat lysate. Lane 1: 1:4000, lane 2: 1:8000, lane 3: 1:12000 dilution of anti-XIAP.

Immunofluorescent staining of Rabbit Liver tissue with anti-XIAP

Publications

Product: Duckett, Nava, Gedrich et al.: "A conserved family of cellular genes related to the baculovirus iap gene and encoding apoptosis inhibitors." in: The EMBO journal, Vol. 15, Issue 11, pp. 2685-94, 1996 (PubMed).

Tamm, Kornblau, Segall et al.: "Expression and prognostic significance of IAP-family genes in human cancers and myeloid leukemias." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 6, Issue 5, pp. 1796-803, 2000 (PubMed).

Fulda, Meyer, Debatin: "Metabolic inhibitors sensitize for CD95 (APO-1/Fas)-induced apoptosis by down-regulating Fas-associated death domain-like interleukin 1-converting enzyme inhibitory protein expression." in: Cancer research, Vol. 60, Issue 14, pp. 3947-56, 2000 (PubMed).

Martins, Iaccarino, Tenev et al.: "The serine protease Omi/HtrA2 regulates apoptosis by binding XIAP through a reaper-like motif." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 439-44, 2002 (PubMed).

Deng, Lin, Wu: "TRAIL-induced apoptosis requires Bax-dependent mitochondrial release of Smac/DIABLO." in: Genes & development, Vol. 16, Issue 1, pp. 33-45, 2002 (PubMed).