p62 Lck Ligand (AA 257-437) antibody

Details for Product No. ABIN968202, Supplier: Log in to see
AA 257-437
Human, Mouse (Murine), Rat (Rattus)
Clonality (Clone)
Monoclonal ()
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen Human p62 lck ligand aa. 257-437
Clone 3-P62 LCK LIGAND
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Background P62 lck ligand (zeta-interacting protein (ZIP)) is a cytoplasmic protein that binds to the SH2 domain of lck (a T cell src tyrosine kinase) in the absence of a phosphotyrosine in either protein. The ubiquitously expressed p62 lck ligand contains a cysteine rich region that is similar to a zinc finger domain, a G protein binding region, a PEST sequence, and several phosphorylation sites. Deletion of the p62 lck ligand N-terminal domain has been reported to abrogate its binding to lck. However, mutation of the tyrosine did not have an effect. In addition, p62 lck ligand binds to the pseudosubstrate region of the PKCzeta catalytic domain. In turn, PKCzeta phosphorylates p62. p62 lck ligand binds to the dimerization region of PKCzeta, thereby inhibiting PKCzeta-PKCzeta interaction. This suggests that p62 lck ligand may compete with PKCzeta. However, it requires PKCzeta for proper subcellular localization. These data suggest that p62 lck ligand may be part of the protein bridge that links PKCzeta to the tyrosine kinases involved in signaling pathways. This antibody is routinely tested by western blot analysis.
Synonyms: Zeta Interacting Protein (ZIP)
Molecular Weight 62 kDa

Related Products: ABIN968551, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-p62 Lck Ligand (AA 257-437) antibody (ABIN968202) Western blot analysis of p62 Ick ligand on a HCT-8 (human colorectal adenocarcinoma, ...
Immunofluorescence (IF) image for anti-p62 Lck Ligand (AA 257-437) antibody (ABIN968202) Immunofluorescence staining on FHs cells (normal human fetal lung fibroblasts, ATCC H...
Product cited in: Cariou, Perdereau, Cailliau, Browaeys-Poly, Béréziat, Vasseur-Cognet, Girard, Burnol: "The adapter protein ZIP binds Grb14 and regulates its inhibitory action on insulin signaling by recruiting protein kinase Czeta." in: Molecular and cellular biology, Vol. 22, Issue 20, pp. 6959-70, 2002 (PubMed).

Puls, Schmidt, Grawe, Stabel: "Interaction of protein kinase C zeta with ZIP, a novel protein kinase C-binding protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 12, pp. 6191-6, 1997 (PubMed).

Background publications Wooten, Seibenhener, Mamidipudi, Diaz-Meco, Barker, Moscat: "The atypical protein kinase C-interacting protein p62 is a scaffold for NF-kappaB activation by nerve growth factor." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 7709-12, 2001 (PubMed).

Joung, Strominger, Shin: "Molecular cloning of a phosphotyrosine-independent ligand of the p56lck SH2 domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 12, pp. 5991-5, 1996 (PubMed).