Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 109-214) antibody

Details for Product No. ABIN968268
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms CG6988, Dmel\\CG6988, Dpdi, EP(3)1022, PDI, dpdi, pdi, Pdi, Pdi2, mKIAA0994, DSI, ERBA2L, GIT, P4Hbeta, PDIA1, PHDB, PO4DB, PO4HB, PROHB, ERp59, Pdia1, Thbp, PDIR, p55, PDA2, PDIP, XPDIp
Epitope
AA 109-214
(14), (14), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine)
(93), (46), (41), (22), (14), (8), (8), (7), (5), (5), (4), (4), (3), (3), (2), (2), (1)
Host
Mouse
(93), (23)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(10), (8), (7), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(89), (45), (31), (24), (23), (22), (15), (13), (10), (9), (4), (3), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 150 μg
Options
Shipping to United States (Change)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN968268
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Bovine PDI
Clone L10CC10
Isotype IgG1
Cross-Reactivity Human, Dog (Canine), Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name PDI
Background The ER is the site of translation of membrane and secretory proteins. Following synthesis, it shuttles these proteins through a contiguous membrane system to their appropriate destinations. Protein disulfide isomerase (PDI) is an abundant, multifunctional, eukaryotic protein. Although it exhibits ubiquitous expression, it is primarily located in the ER lumen. Here, it functions to catalyze the isomerization of intramolecular disulfide bridges, thereby allowing them to generate their most thermodynamically stable configurations. This role in rearrangement has lead to the classification of PDI as a chaperone. Although protein folding occurs in its absence, PDI may be essential for it to proceed at a physiological relevant rate. In addition, PDI is the beta-subunit of prolyl 4- hydroxylase and is a component of the triglyceride transfer complex. PDI is retained in the ER lumen via its C-terminal -KDEL sequence. Via this sequence, it is continuously recycled back to the ER from other membranous compartments. Thus, PDI is a diverse protein whose primary function may be to correct disulfide bonding and, thus, ensure the most stable conformation of newly synthesized proteins.
Molecular Weight 55 kDa
Research Area Cancer, Organelles
Comment

Related Products: ABIN968551, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 109-214) antibody Western blot analysis of PDI on HCT-8 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-PDI antibdy.
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 109-214) antibody (2) anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 109-214) antibody (Image 2)
Product cited in: Wetterau, Combs, Spinner et al.: "Protein disulfide isomerase is a component of the microsomal triglyceride transfer protein complex." in: The Journal of biological chemistry, Vol. 265, Issue 17, pp. 9800-7, 1990 (PubMed).

Yamauchi, Yamamoto, Hayashi et al.: "Sequence of membrane-associated thyroid hormone binding protein from bovine liver: its identity with protein disulphide isomerase." in: Biochemical and biophysical research communications, Vol. 146, Issue 3, pp. 1485-92, 1987 (PubMed).

Weissman, Kim: "Efficient catalysis of disulphide bond rearrangements by protein disulphide isomerase." in: Nature, Vol. 365, Issue 6442, pp. 185-8, 1993 (PubMed).

Schlegel, Arvan, Lisanti: "Caveolin-1 binding to endoplasmic reticulum membranes and entry into the regulated secretory pathway are regulated by serine phosphorylation. Protein sorting at the level of the endoplasmic reticulum." in: The Journal of biological chemistry, Vol. 276, Issue 6, pp. 4398-408, 2001 (PubMed).

Jenne, Frey, Brugger et al.: "Oligomeric state and stoichiometry of p24 proteins in the early secretory pathway." in: The Journal of biological chemistry, Vol. 277, Issue 48, pp. 46504-11, 2002 (PubMed).

Validation Images
Did you look for something else?
back to top