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PDI antibody (Prolyl 4-Hydroxylase, beta Polypeptide) (AA 109-214)

Details for Product anti-P4HB Antibody No. ABIN968268, Supplier: Login to see New
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Synonyms CG6988, Dmel\\CG6988, Dpdi, EP(3)1022, PDI, dpdi, pdi, Pdi, Pdi2, mKIAA0994, DSI, ERBA2L, GIT, P4Hbeta, PDIA1, PHDB, PO4DB, PO4HB, PROHB, ERp59, Pdia1, Thbp, PDIR, p55, PDA2, PDIP, XPDIp
AA 109-214
(17), (10), (7), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(148), (80), (73), (25), (24), (22), (22), (21), (20), (20), (20), (18), (3), (3), (2), (2), (1)
(148), (44)
Clonality (Clone)
Monoclonal ()
This PDI antibody is un-conjugated
(14), (14), (14), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Immunofluorescence (IF), Western Blotting (WB)
(147), (73), (70), (70), (55), (54), (30), (12), (12), (8), (6), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Quantity 150 μg
Shipping to United States ( )
Immunogen Bovine PDI aa. 109-214
Clone 34-PDI
Isotype IgG1
Cross-Reactivity Human, Dog (Canine), Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name PDI (P4HB Antibody Abstract)
Background The ER is the site of translation of membrane and secretory proteins. Following synthesis, it shuttles these proteins through a contiguous membrane system to their appropriate destinations. Protein disulfide isomerase (PDI) is an abundant, multifunctional, eukaryotic protein. Although it exhibits ubiquitous expression, it is primarily located in the ER lumen. Here, it functions to catalyze the isomerization of intramolecular disulfide bridges, thereby allowing them to generate their most thermodynamically stable configurations. This role in rearrangement has lead to the classification of PDI as a chaperone. Although protein folding occurs in its absence, PDI may be essential for it to proceed at a physiological relevant rate. In addition, PDI is the beta-subunit of prolyl 4- hydroxylase and is a component of the triglyceride transfer complex. PDI is retained in the ER lumen via its C-terminal -KDEL sequence. Via this sequence, it is continuously recycled back to the ER from other membranous compartments. Thus, PDI is a diverse protein whose primary function may be to correct disulfide bonding and, thus, ensure the most stable conformation of newly synthesized proteins.
Molecular Weight 55 kDa
Research Area Cancer, Organelles

Related Products: ABIN968551, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-PDI antibody (Prolyl 4-Hydroxylase, beta Polypeptide) (AA 109-214) (ABIN968268) Western blot analysis of PDI on HCT-8 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane...
 image for anti-PDI antibody (Prolyl 4-Hydroxylase, beta Polypeptide) (AA 109-214) (ABIN968268) anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 109-214) antibody (Image 2)
Western Blotting (WB) image for anti-PDI antibody (Prolyl 4-Hydroxylase, beta Polypeptide) (AA 109-214) (ABIN968268) anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 109-214) antibody (Image 3)
Product cited in: Jenne, Frey, Brugger et al.: "Oligomeric state and stoichiometry of p24 proteins in the early secretory pathway." in: The Journal of biological chemistry, Vol. 277, Issue 48, pp. 46504-11, 2002 (PubMed).

Schlegel, Arvan, Lisanti: "Caveolin-1 binding to endoplasmic reticulum membranes and entry into the regulated secretory pathway are regulated by serine phosphorylation. Protein sorting at the level of the endoplasmic reticulum." in: The Journal of biological chemistry, Vol. 276, Issue 6, pp. 4398-408, 2001 (PubMed).

Weissman, Kim: "Efficient catalysis of disulphide bond rearrangements by protein disulphide isomerase." in: Nature, Vol. 365, Issue 6442, pp. 185-8, 1993 (PubMed).

Yamauchi, Yamamoto, Hayashi et al.: "Sequence of membrane-associated thyroid hormone binding protein from bovine liver: its identity with protein disulphide isomerase." in: Biochemical and biophysical research communications, Vol. 146, Issue 3, pp. 1485-92, 1987 (PubMed).

Wetterau, Combs, Spinner et al.: "Protein disulfide isomerase is a component of the microsomal triglyceride transfer protein complex." in: The Journal of biological chemistry, Vol. 265, Issue 17, pp. 9800-7, 1990 (PubMed).

Catalog No. ABIN968268
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