Dynactin p50 (AA 55-196) antibody

Details for Product No. ABIN968308, Supplier: Log in to see
AA 55-196
Fly (Calliphora), Human, Mouse (Murine), Rat (Rattus)
Clonality (Clone)
Monoclonal ()
Immunofluorescence (IF), Western Blotting (WB)
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Immunogen Human Dynactin p50 aa. 55-196
Clone 25-Dynactin p50
Isotype IgG1
Cross-Reactivity Rat (Rattus), Mouse (Murine), Insect
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Background Dyneins are ubiquitous, multimeric proteins that are responsible for minus end-directed microtubule-based organelle transport. They are divided into two classes, cytosolic and axonemal. The activity of cytosolic dynein is regulated by the multiprotein dynactin complex with which it co-purifies. Dynactin is composed of at least nine polypeptides with p50 being the second most abundant subunit. The complex consists of an F-actin-like core filament of actin-related protein Arp1, a heterodimer that acts as a cap at one end, and a 62kDa subunit at the other end. p150[glued] is the largest subunit of the complex. The precise function of dynactin remains to be determined, but it may be recruited to prometaphase kinetochores to assist in alignment and spindle organization during mitosis. Additionally, dynactin localizes at the tail of cytosolic dynein and activates dynein-mediated vesicle movement. Therefore, dynactin may function to anchor or target dynein to the kinetochore during mitosis.
Molecular Weight 50 kDa

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Dynactin p50 (AA 55-196) antibody (ABIN968308) Western blot analysis of Dynactin p50 on Jurkat cell lysate. Lane 1: 1:250, lane 2: 1...
Immunofluorescence (IF) image for anti-Dynactin p50 (AA 55-196) antibody (ABIN968308) Immunofluorescent staining of WIDR cells.
Product cited in: Kuo, Liu, Wei, Lin-Lee, Tatebe, Mills, Unate: "Induction of human MDR1 gene expression by 2-acetylaminofluorene is mediated by effectors of the phosphoinositide 3-kinase pathway that activate NF-kappaB signaling." in: Oncogene, Vol. 21, Issue 13, pp. 1945-54, 2002 (PubMed).

Holleran, Ligon, Tokito, Stankewich, Morrow, Holzbaur: "beta III spectrin binds to the Arp1 subunit of dynactin." in: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36598-605, 2001 (PubMed).

Starr, Williams, Hays, Goldberg: "ZW10 helps recruit dynactin and dynein to the kinetochore." in: The Journal of cell biology, Vol. 142, Issue 3, pp. 763-74, 1998 (PubMed).

Burkhardt, Echeverri, Nilsson, Vallee: "Overexpression of the dynamitin (p50) subunit of the dynactin complex disrupts dynein-dependent maintenance of membrane organelle distribution." in: The Journal of cell biology, Vol. 139, Issue 2, pp. 469-84, 1997 (PubMed).

Echeverri, Paschal, Vaughan, Vallee: "Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 617-33, 1996 (PubMed).

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