PI3-Kinase p170 antibody
Western Blotting (WB), Immunofluorescence (IF)
|2 references available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
|Immunogen||Mouse PI3-Kinase p170|
|Cross-Reactivity||Rat (Rattus), Human|
|Description||PI3-kinase phosphorylates the D-3 position of the inositol ring of phosphatidylinositol (PtdIns), producing PtdIns(3)P, PtdIns(3,4)P2, and PtdIns(3,4,5)P3. PI3-Kinase is a heterodimer of an 85 kDa regulatory subunit (p85) and a 110 kDa catalytic subunit (p110). However, this heterodimer is only one member of a larger family of proteins with similarity to the p110 subunit. These different PI3-kinase isoforms have been divided into three classes. Class I consists of p110alpha and p110beta which bind the p85 subunit and associate with receptor tyrosine kinases. Class II contains 68D, cpk, and p170. These proteins all contain a C-terminal C2 domain and phosphorylate PtdIns and PtdIns(4)P, but not PtdIns(4,5)P2. Class III members only phosphorylate PtdIns to PtdIns(3)P and include the S. cerevisiae Vps34p and its human homologs. The first human Class II PI3-kinase to be described, p170, contains the characteristic C-terminal phosphoinositide-binding C2 domain also found in PKC isoforms and synaptotagmins. In addition, expression of PI3-kinase p170 is widespread and may indicate that the protein performs a housekeeping function in cell growth and differentiation.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||170 kDa|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
|Western blot analysis of PI3-Kinase p170 on rat brain lysate. Lane 1: 250, lane 2: 1:500, lane 3: 1:1000 dilution of PI3-Kinase p170. WI38 anti-PI3-Kinase p170 antibody (Image 3)|
Virbasius, Guilherme, Czech: "Mouse p170 is a novel phosphatidylinositol 3-kinase containing a C2 domain." in: The Journal of biological chemistry, Vol. 271, Issue 23, pp. 13304-7, 1996 (PubMed).
Brown, Ho, Weber-Hall et al.: "Identification and cDNA cloning of a novel mammalian C2 domain-containing phosphoinositide 3-kinase, HsC2-PI3K." in: Biochemical and biophysical research communications, Vol. 233, Issue 2, pp. 537-44, 1997 (PubMed).