SRSF Protein Kinase 1 (SRPK1) (AA 312-434) antibody

Details for Product No. ABIN968353
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Antigen
Synonyms BEST:CK01209, CG11489, CG9085, CK01209, Dmel\\CG11489, SRPK, SRPK1, Srpk79D, atc, srpk1, zgc:63789, wu:fc06h03, AU017960, SFRSK1
Epitope
AA 312-434
(18), (12), (9), (6), (4), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(75), (23), (23), (12), (12), (12)
Host
Mouse
(60), (15)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(64), (48), (12), (10), (9), (8), (4), (4), (2)
Pubmed 2 references available
Quantity 50 μg
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Catalog No. ABIN968353
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Immunogen Human SRPK1
Clone 6G3
Isotype IgG1
Cross-Reactivity Dog (Canine), Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name SRPK1
Background Mammalian cell pre-mRNA splicing is mediated by the spliceosome, a multi-component complex that contains two types of splicing factors: small nuclear ribonucleoprotein particles (snRNPs) and non-snRNP factors. Interactions between snRNPs and pre-mRNA ensures proper establishment of a catalytic core for the splicing reaction. However, these interactions are mediated by the non-snRNP factors. The super family of Arg/Ser-rich (RS) domain containing splicing factors are well known non-snRNPs. All of these proteins share a similar structure consisting of an N-terminal RNA recognition motif and a C-terminal RS domain. However, different SR factors have distinct specificities and function is regulated by their level of expression and by reversible phosphorylation. Two families of kinases phosphorylate SR domain-containing proteins: SR protein-specific kinases (SRPK1 and 2) and Clk/Sty. SRPL1 is specific for SR domain-containing splicing factors because it recognizes only Arg and phosphorylates only Ser. SRPK1 is expressed predominately in the pancreas, domain-containing splicing factors because it recognizes only Arg and phosphorylates only Ser. SRPK1 is expressed predominately in the pancreas, whereas SRPK2 is highly expressed in brain. SRPKs affect splice-site selection and are thought to affect alternative splicing.
Molecular Weight 92 kDa
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-SRSF Protein Kinase 1 (SRPK1) (AA 312-434) antibody Immunofluorescent staining of EaHy cells.
anti-SRSF Protein Kinase 1 (SRPK1) (AA 312-434) antibody (2) Western blot analysis of SRPK1 on HeLa cell lysate. Lane 1:1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-SRPK1.
anti-SRSF Protein Kinase 1 (SRPK1) (AA 312-434) antibody (3) anti-SRSF Protein Kinase 1 (SRPK1) (AA 312-434) antibody (Image 3)
Product cited in: Colwill, Feng, Yeakley et al.: "SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 24569-75, 1996 (PubMed).

Wang, Lin, Dyck et al.: "SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells." in: The Journal of cell biology, Vol. 140, Issue 4, pp. 737-50, 1998 (PubMed).

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